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, Volume 10, Issue 4, pp 329–334 | Cite as

Determination of kallikrein by radioimmunoassay in human body fluids

  • Klaus Mann
  • Berthold Lipp
  • Joachim Grunst
  • Reinhard Geiger
  • Hans Jörg Karl
Histamine and Kinins

Abstract

A sensitive and specific radioimmunoassay for human urinary kallikrein was developed, which allows tissue kallikrein determination in human urine, saliva, pancreatic juice, bile and sweat. In several body fluids a kallikrein-like antigen was found, but not in gastric juice and breast milk. According to gel filtration studies, complex formation of kallikrein with serum proteins or different molecular weight forms of kallikrein in serum and urine may be assumed. Pancreatic kallikrein secretion follows the same pattern after stimulation with secretin and cholecystokinin as trypsin and chymotrypsin in normal individuals. In chronic pancreatitis the kinetic behaviour remains unchanged with respect to the enzyme secretion, but the secretion of kallikrein is reduced to about 20%.

Keywords

Pancreatitis Chronic Pancreatitis Body Fluid Breast Milk Secretin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Klaus Mann
    • 1
  • Berthold Lipp
    • 1
  • Joachim Grunst
    • 1
  • Reinhard Geiger
    • 2
  • Hans Jörg Karl
    • 1
  1. 1.Medizinische Klinik II, Klinikum GroßhadernUniversität MünchenFRG
  2. 2.Abteilung für Klinische Chemie und Klinische Biochemie in der Chirurgischen KlinikUniversität MünchenFRG

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