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Decrease in peritoneal mast cell count in rats with adjuvant arthritis. II. inhibition by anti-inflammatory and immunosuppressive drugs

Agents and Actions volume 10, pages 317–321 (1980)Cite this article

Abstract

The following drugs were administered daily to adjuvant-injected rats: indomethacin, flufenamic acid, phenylbutazone, prednisolone, dexamethasone, methotrexate and 6-mercaptopurine. The effects on the decrease in the mast-cell count and histamine content of the peritoneal fluid were studied as well as the effect on the development of polyarthritis 14 days after adjuvant injection. At the doses tested, these drugs markedly inhibited changes in the parameters of peritoneal fluid. With the exception of flufenamic acid, the preventive effects on the decrease in the peritoneal mast-cell count paralleled the prevention of development of arthritic lesions. 6-Mercaptopurine administered daily for 5 consecutive days starting from day 0 was almost as effective as when it was given over the entire period (days 0 through 13).

These results show that the decrease in the peritoneal mast-cell count is one of the symptoms of adjuvant disease in rats that can be used for the quantitative evaluation of antirheumatic drugs.

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References

  1. S. Seo, J. Yokoyama andK. Saeki,Decrease in Peritoneal Mast-Cell Count in Rats with Adjuvant Arthritis. I. Time Course and Interstrain Difference, Agents and Actions10, 309–316 (1980).

    PubMed  Google Scholar 

  2. B.H. Waksman, C.M. Pearson andJ.T. Sharp,Studies of Arthritis and Other Lesions Induced in Rats by Injection of Mycobaterial Adjuvant. II. Evidence that the Disease is a Disseminated Immunologic Response to Exogenous Antigen. J. Immunol.85, 403–417 (1960).

    PubMed  Google Scholar 

  3. C.M. Pearson, B.H. Waksman andJ.T. Sharp,Studies of Arthritis and Other Lesions Induced in Rats by Injection of Mycobacterial Adjuvant. V. Changes Affecting Skin and Mucous Membranes. Comparison of the Experimental Process with Human Disease. J. Exp. Med.113, 485–510 (1961).

    PubMed  Google Scholar 

  4. B.H. Waksman andC. Wennersten,Passive Transfer of Adjuvant Arthritis in Rats with Living Lymphoid Cells of Sensitized Donors. Int. Arch. Allergy Appl. Immunol.23, 129–139 (1963).

    PubMed  Google Scholar 

  5. C.G. Van Arman,Pathway to Adjuvant Arthritis, Fed. Proc.35, 2442–2446 (1976).

    PubMed  Google Scholar 

  6. B.B. Newbould,Chemotherapy of Arthritis Induced in Rats by Mycobacterial Adjuvant, Br. J. Pharmacol.21, 127–136 (1963).

    PubMed  Google Scholar 

  7. J.R. Ward, R.S. Cloud, E.L. Krawitt andR.S. Jones,Studies on Adjuvant-Induced Polyarthritis in Rats. III. The Effect of ‘Immunosuppressive Agents’ on Arthritis and Tuberculin Hypersensitivity, Arthritis Rheum.7, 654–661 (1964).

    Google Scholar 

  8. M.L. Graeme, E. Fabry andE.B. Sigg,Mycobaterial Adjuvant Periarthritis in Rodents and its Modification by Anti-inflammatory Agents. J. Pharmac. exp. Ther.153, 373–380 (1966).

    Google Scholar 

  9. J.R. Ward andR.S. Cloud,Comparative Effect of Anti-Rheumatic Drugs on Adjuvant-induced Polyarthritis in Rats. J. Pharmac. exp. Ther.152, 116–121 (1966).

    Google Scholar 

  10. C.A. Winter andG.W. Nuss,Treatment of Adjuvant Arthritis in Rats with Anti-Inflammatory Drugs, Arthritis Rheum.9, 394–404 (1966).

    PubMed  Google Scholar 

  11. D.T. Walz, M.J. DiMartino andA. Misher,Adjuvant-Induced Arthritis in Rats. II. Drug Effects on Physiologic, Biochemical and Immunologic Parameters, J. Pharmac. exp. Ther.178, 223–231 (1971).

    Google Scholar 

  12. K. Saeki, S. Muraoka andH. Yamasaki,Anti-Inflammatory Properties of N-Phenylanthranilic Acid Derivatives in Relation to Uncoupling of Oxidative Phosphorylation, Jap. J. Pharmacol.22, 187–199 (1972).

    PubMed  Google Scholar 

  13. M.E. Rosenthale,Evaluation for Immunosuppressive and Antiallergic Activity, in:Antiinflammatory Agents; Chemistry and Pharmacology, vol. 2 (Eds. R.A. Scherrer and M.W. Whitehouse; Academic Press, New York 1974), pp. 123–192.

    Google Scholar 

  14. F. Quagliata, P.M. Sanders andD.L. Gardner,Suppression of Adjuvant Arthritis by a New Cytotoxic Compound, Rubidomycin, Ann. Rheum. Dis.28, 163–171 (1969).

    PubMed  Google Scholar 

  15. A.R. Page, R.M. Condie andR.A. Good,Effect of 6-Mercaptopurine on Inflammation, Am. J. Pathol.40, 519–530 (1962).

    PubMed  Google Scholar 

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  1. Department of Pharmacology, Okayama University Medical School, 2-5-1 Shikata-cho, 700, Okayama, Japan

    Saiichirou Seo

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Seo, S. Decrease in peritoneal mast cell count in rats with adjuvant arthritis. II. inhibition by anti-inflammatory and immunosuppressive drugs. Agents and Actions 10, 317–321 (1980). https://doi.org/10.1007/BF01971431

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  • DOI: https://doi.org/10.1007/BF01971431

Keywords

  • Arthritis
  • Mast Cell
  • Histamine
  • Dexamethasone
  • Methotrexate