Decreased tissue immunoregulatory index in rheumatoid synovitis, determined by in-situ T-cell phenotyping with monoclonal antibodies
- 16 Downloads
The phenotypic tissue immunoregulatory index in rheumatoid synovitis is locally different and varies significantly between given areas. Ectopic lymphoid follicles show normal to high T4∶T8 ratios, whereas within the sublining perivascular and diffuse infiltrate T4∶T8 ratios are decreased. Thus the frequency reported immunoregulatory indices of eluted cells can't be representative for an immunoregulatory tissue disequilibrium in rheumatoid arthritis. An elevated T4∶T8 ratio in eluted cells may correlate with a follicular B-cell hyperplasia in rheumatoid synovitis (low local activity), a low T4∶T8 ratio may be related to rheumatoid synovitis with diffuse sublining T-cell infiltrates (high local activity). Our results are in principal accordance with the findings of Kurosaka and Ziff  and Meijer et al. . They also found different T-cell distributions in synovial tissue depending on the histopathological localization.
For these topographical reasons, immunoregulatory indices determined in eluted cells from tissue samples cannot be representative for the immunopathological reactions at the synovial membrane tissue level.
The decrease in the phenotypic tissue immunoregulatory index in the rheumatoid synovium from deeper to superficial areas may be explained as a compensatory influx of peripheral, functionally defect T8-cytotoxic/suppressor cells (see Fig. 2).
KeywordsLocal Activity Synovial Membrane Tissue Meijer Lymphoid Follicle Rheumatoid Synovium
Unable to display preview. Download preview PDF.
- G. C. Faure, M. A. Bach, M. C. Bene, M. F. Kahn, H. A. Gaucher and J. F. Bach,T-cell subsets in peripheral blood from rheumatoid arthritis people. XV. Int. Congress of Rheumatology, Paris 1981, abstract 72.Google Scholar
- P. H. Hermans, E. M. Veys, G. Goldstein, P. Kung, J. Schindler, J. Symoens and J. van Wauwe,Analysis of the T-cell populations determined by monoclonal antibodies in rheumatoid arthritis. XV. Int. Congress of Rheumatology, Paris 1981, abstract 73.Google Scholar
- M. Duclos, H. Zeidler, Liman, W. L. Pichler, P. Rieber and H. H. Peter,Characterization of blood and synovial fluid lymphocytes from patients with rheumatoid arthritis and other joint diseases by monoclonal antibodies (OKT series) and acid-alpha-naphtylesterase staining. Rheumatol. Int.2, 75–82 (1982).CrossRefPubMedGoogle Scholar
- F. Raeman, W. De Cock, F. Leempoels, J. De Cree and H. Verhaegen,Enumeration of T-lymphocytes and T-lymphocyte subsets in rheumatoid arthritis using monoclonal antibodies. Clin. Rheumatol.1 (1981) (abstract).Google Scholar
- P. Lydyard, A. Hanglow, I. Hartley, A. Young and I. M. Roitt,Immunoregulation in patients with rheumatoid arthritis. Lancet799 (1982).Google Scholar
- A. Ranke, T. Paavonen, E. Tolvanen, Y. Konttinen, E. Kankaanpäa, Nilson and G. Biberfeld,Suppressor and helper T cells in rheumatoid synovia XV. Congress of Rheumatology, Paris 1981, abstract 70.Google Scholar
- J. G. Saal, H. Rautenstrauch, P. Fritz, W. Laschner and M. R. Hadam,T-lymphocytes with Fc-receptors for IgG are highly enriched in synovium of rheumatoid arthritis. XV. Congress of Rheumatology, Paris 1981, abstract 71.Google Scholar
- R. I. Fox, T. C. Adamson, D. Frisman, I. Royston and J. H. Vaughan,Identification of lymphocytes infiltrating synovial membrane in rheumatoid arthritis. Arthritis Rheum.25, 50 (1982) (abstract).Google Scholar
- R. Dreher and K. Federlin,Die Bedeutung der funktionellen Morphologie, Immunoregulation und-hämostasiologie für die Klinik der rheumatoiden Arthritis. 88. Tagung Dtsch. Ges. Innere Med. Wiesbaden, April 1982Google Scholar
- R. Dreher, U. Weber, B. Rohrbach, G. Köhler, W. Mohing and K. Federlin,Histologische Synoviaanalyse mit monoklonalen Antikörpern. Zschr. Rheumatologie41, 4, 120–121 (1982).Google Scholar
- R. Dreher,Experimentelle Immunarthritis und ihre Bedeutung in der Rheumatherapieforschung und Rheumapathologie. Werksverlag Dr. E. Banaschewski, München-Gräfelfing 1983.Google Scholar