High pressure liquid chromatographic analysis of the serum concentration of cefuroxime after an intravenous bolus injection of cefuroxime in patients with a coronary artery bypass grafting
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A simple reversed-phase high pressure liquid chromatographic method was developed for the determination of cefuroxime in the serum of patients undergoing coronary artery bypass grafting. The serum was cleaned up with a 3.3% solution of perchloric acid in water.
Cefalexine was used as an internal standard. Detection was made by a UV multi-wavelength detector. The optimum wavelength for cefuroxime is 275 nm. The absolute recovery of this method was 90.9%; the limit of quantification was 0.7 mg/l. This analytical method was used in a study to investigate the cefuroxime serum concentration-time curves in 26 patients undergoing coronary artery bypass grafting. It was found that one single dose is sufficient to obtain effective serum concentrations.
KeywordsCefuroxime Chromatography, high pressure liquid Pharmacokinetics
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- 1.Scheld WM, Sande MA. Endocarditis and intravascular infections. In: Mandell GL, Douglas RG, Bennett JE, eds. Principles and practice of infectious disease. New York: Churchill Livingstone, 1990:670–706.Google Scholar
- 3.Brogard JM, Kopferschmitt JK, Pinget M, Arnaud JP, Lavillaureix J. Cefuroxime concentrations in serum, urine and bile: pharmacokinetic profile. Proc R Soc Med 1977;70:42–50.Google Scholar
- 4.O'Callaghan CH, Harding SM. The pharmacokinetics of cefuroxime in man in relation to its anti-bacterial activity. Proc R Soc Med 1977;70:4–10.Google Scholar
- 6.Bundtzen RW, Toothaker RD, Nielson OS, Madsen PO, Welling PG, Craig WA. Pharmacokinetics of cefuroxime in normal and impaired renal function: comparison of high-pressure liquid chromatography and microbiological assays. Antimicrob Agents Chemother 1981;3:443–9.Google Scholar
- 10.Hekster YA, Baars AM, Vree TB, Van Klingeren B, Rutgers A. Comparison of the determination ofβ-lactam antibiotic drugs in plasma of rabbits by means of HPLC and of a microbiological assay. Pharm Weekbl [Sci] 1979;1:95–100.Google Scholar