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Pharmaceutisch Weekblad

, Volume 14, Issue 6, pp 360–364 | Cite as

High pressure liquid chromatographic analysis of the serum concentration of cefuroxime after an intravenous bolus injection of cefuroxime in patients with a coronary artery bypass grafting

  • M. J. Koot
  • F. N. Ijdenberg
  • R. M. Stuurman
  • J. Poell
  • L. J. Bras
  • J. J. M. Langemeijer
  • L. Huen
Practice/Case Reports

Abstract

A simple reversed-phase high pressure liquid chromatographic method was developed for the determination of cefuroxime in the serum of patients undergoing coronary artery bypass grafting. The serum was cleaned up with a 3.3% solution of perchloric acid in water.

Cefalexine was used as an internal standard. Detection was made by a UV multi-wavelength detector. The optimum wavelength for cefuroxime is 275 nm. The absolute recovery of this method was 90.9%; the limit of quantification was 0.7 mg/l. This analytical method was used in a study to investigate the cefuroxime serum concentration-time curves in 26 patients undergoing coronary artery bypass grafting. It was found that one single dose is sufficient to obtain effective serum concentrations.

Keywords

Cefuroxime Chromatography, high pressure liquid Pharmacokinetics 

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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1992

Authors and Affiliations

  • M. J. Koot
    • 1
  • F. N. Ijdenberg
    • 1
  • R. M. Stuurman
    • 1
  • J. Poell
    • 2
  • L. J. Bras
    • 2
  • J. J. M. Langemeijer
    • 2
  • L. Huen
    • 1
  1. 1.Department of Clinical PharmacySt. Antonius HospitalEM Nieuwegeinthe Netherlands
  2. 2.Department of AnaesthesiologySt. Antonius Hospitalthe Netherlands

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