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Pharmaceutisch Weekblad

, Volume 14, Issue 6, pp 353–356 | Cite as

Antimicrobial screening of essential oils and extracts of someHumulus lupulus L. cultivars

  • C. R. Langezaal
  • A. Chandra
  • J. J. C. Scheffer
Articles

Abstract

The essential oils as well as solvent extracts of 11 hop cultivars, 1 hop variety and a wild type of hop were screened for their antimicrobial activities using the agar overlay technique. The oils were isolated from the cones of the various hop plants by hydrodistillation, the extracts were obtained by soaking the hop cones in chloroform. The oils and the extracts showed activity against the Gram-positive bacteria (Bacillus subtilis andStaphylococcus aureus) and the fungus (Trichophyton mentagrophytes var.interdigitale), but almost no activity against the Gram-negative bacterium (Escherichia coli) and the yeast (Candida albicans) used in the screening. The peak area percentages of the main volatile components and the contents of the bitter acids of the extracts were determined for all cultivars using chromatographic methods.

Keywords

Agar overlay technique Antibiotics Drug screening Essential oil Humulus lupulus L. 

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References

  1. 1.
    Verhagen LC. Hop analysis. In: Linskens HF, Jackson JF, eds. Modern methods of plant analysis. New series. Vol. 7. Beer analysis. Heidelberg: Springer-Verlag, 1988:67–87.Google Scholar
  2. 2.
    Vanhoucke LC. De folklore van de hop in Vlaams-België vroeger en nu [The folklore of hop in Flemish Belgium in the past and at present]. Oudenaarde: Drukkerij Sanderus, 1964.Google Scholar
  3. 3.
    Boatwright J. Antimicrobial activity of hop oil emulsion. J Inst Brew 1976;82:334–5.Google Scholar
  4. 4.
    Mizobuchi S, Sato Y. A new flavone with antifungal activity isolated from hops. Agric Biol Chem 1984;48:2771–5.Google Scholar
  5. 5.
    Mizobuchi S, Sato Y. Antifungal activities of hop bitter resins and related compounds. Agric Biol Chem 1985;49:399–403.Google Scholar
  6. 6.
    Mizobuchi S, Sato Y. Antifungal activities of 2,4,6-trihydroxyacylophenones and related compounds. Agric Biol Chem 1985;49:719–24.Google Scholar
  7. 7.
    Mizobuchi S, Sato Y. Antifungal activity of 2,4-dihydroxyacylophenones and related compounds. Agric Biol Chem 1985;49:1327–33.Google Scholar
  8. 8.
    European Pharmacopoeia. Vol. 3. Sainte-Ruffine: Maisonneuve SA, 1975:68–71.Google Scholar
  9. 9.
    Van den Dries JMA, Baerheim Svendsen A. A simple method for detection of glycosidic bound monoterpenes and other volatile compounds occurring in fresh plant material. Flavour Fragr J 1989;4:59–61.Google Scholar
  10. 10.
    Katsiotis ST, Langezaal CR, Scheffer JJC, Verpoorte R. Comparative study of the essential oils from hops of variousHumulus lupulus L. cultivars. Flavour Fragr J 1989;4:187–91.Google Scholar
  11. 11.
    Katsiotis ST, Langezaal CR, Scheffer JJC. Composition of the essential oils from leaves of variousHumulus lupulus L. cultivars. Flavour Fragr J 1990;5:97–100.Google Scholar
  12. 12.
    Janssen AM. Antimicrobial activities of essential oils — a pharmacognostical study [dissertation]. Leiden: Univ of Leiden, 1989.Google Scholar
  13. 13.
    Teuber M, Schmalreck AF. Membrane leakage inBacillus subtilis 168 induced by the hop constituents lupulone, humulone, isohumulone and humulinic acid. Arch Mikrobiol 1973;94:159–71.CrossRefPubMedGoogle Scholar
  14. 14.
    Schmalreck AF, Teuber M, Reininger W, Hartl A. Structural features determining the antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives. Can J Microbiol 1975;21: 205–12.PubMedGoogle Scholar
  15. 15.
    Van Sumere CF, Vande Casteele K, Hutsebaut W, Everaert E, De Cooman L, Meulemans W. RP-HPLC analysis of flavonoids and the biochemical identification of hop cultivars. EBC Monograph XIII. Nürnberg: Carl (Brauwelt-Verlag), 1987:146–75.Google Scholar
  16. 16.
    Janssen AM, Chin NLJ, Scheffer JJC, Baerheim Svendsen A. Screening for antimicrobial activity of some essential oils by the agar overlay technique. Pharm Weekbl [Sci] 1986;8:289–92.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1992

Authors and Affiliations

  • C. R. Langezaal
    • 1
  • A. Chandra
    • 1
  • J. J. C. Scheffer
    • 1
  1. 1.Division of Pharmacognosy, Center for Bio-Pharmaceutical SciencesLeiden University, Gorlaeus LaboratoriesRA Leidenthe Netherlands

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