Experientia

, Volume 36, Issue 6, pp 637–638 | Cite as

Synthesis of L-[3-2H,18O]glycerol and its incorporation into the 4-methyl-5-hydroxyethyl thiazole moiety of thiamine byEscherichia coli

  • R. H. White
Article

Summary

L-[3-2H,18O]glycerol was prepared and fed toEscherichia coli in order to determine the origin of the oxygen atom in the biosynthesized thiazole moiety of thiamine. Measurement by GC-MS of the isotope incorporation into the thiazole from this substrate confirmed that the 2 hydrogens and the oxygen on the C-3 carbon of glycerol are incorporated directly into the thiazole.

Keywords

Oxygen Hydrogen Glycerol Oxygen Atom Thiamine 

References

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    R.H. White, Biochemistry17, 3833 (1978).CrossRefPubMedGoogle Scholar
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    R.H. White and F.B. Rudolph, Biochim. biophys. Acta542, 340 (1978).PubMedGoogle Scholar
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    E. Baer and H.O.L. Fischer, J. biol. Chem.128, 463 (1939).Google Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • R. H. White
    • 1
  1. 1.Department of BiochemistryRice UniversityHoustonUSA

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