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Purification of canine myocardial mitochondrial creatine kinase

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Summary

Mitochondrial creatine kinase, purified for the first time, is a dimeric molecule with a mol.wt of 82,000 and does not hybridize with M or B subunits or react to their specific antiserum. A specific antiserum to the mitochondrial form was developed which does not cross-react with the B or M subunits. Thus, the mitochondrial form is biochemically and immunologically unique.

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Authors and Affiliations

  1. Cardiovascular Division, Washington University School of Medicine, 660 South Euclid Avenue, 63110, St. Louis, Missouri, USA

    R. Roberts

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  1. R. Roberts
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Additional information

Supported in part by the Special Center of Research in Ischemic Heart Disease (1 P17 HL 17646).

I thank Ann Grace for her technical assistance and Ava Ysaguirre for typing the mansucript.

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Roberts, R. Purification of canine myocardial mitochondrial creatine kinase. Experientia 36, 632–634 (1980). https://doi.org/10.1007/BF01970102

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