Advertisement

Experientia

, Volume 41, Issue 7, pp 946–948 | Cite as

Acetaldehyde oxidation inDrosophila null-mutants for alcohol dehydrogenase

  • F. Garcin
  • C. Larochelle
  • G. Lau You Hin
  • J. Côté
Short Communications Genetics, Developmental Biology

Summary

Aldehyde dehydrogenase (ALDH) activity is demonstrated in four strains ofD. melanogaster lacking active alcohol dehydrogenase (ADH-null mutants). In the four strains, ALDH activities are similar to those found in a wild strain. It is concluded that ADH-null flies are able to detoxify acetaldehyde. This finding is discussed in relation with the dual function of ADH proposed recently.

Key words

Acetaldehyde oxydation alcohol dehydrogenase aldehyde dehydrogenase Drosophila melanogaster Drosophila simulans ethanol catabolism null-mutants for alcohol dehydrogenase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 2.
    McDonald, J.F., Chambers, G.K., David, J., and Ayala, F.J., Proc. natn. Acad. Sci. USA74 (1977) 4562.Google Scholar
  2. 3.
    Moxon, L.N., Holmes, R.S., and Parsons, P.A., Comp. Biochem. Physiol.71B (1982) 387.Google Scholar
  3. 4.
    David, J., Ann. Biol.16 (1977) 451.Google Scholar
  4. 5.
    McKenzie, J.A., and Parsons, P.A., Oecologia (Berl.)10 (1972) 373.Google Scholar
  5. 6.
    Courtright, J.B., Genetics57 (1967) 25.PubMedGoogle Scholar
  6. 7.
    Dickinson, W.J., Devl Biol.26 (1971) 77.Google Scholar
  7. 8.
    David, J., Bocquet, C., Van Herrewege, J., Fouillet, P., and Arens, M.F., Biochem. Genet.16 (1978) 203.PubMedGoogle Scholar
  8. 9.
    Larochelle, C., Côté, J., and Garcin, F., Drosoph. Inf. Serv.60 (1984) in press.Google Scholar
  9. 10.
    Harada, S., Agarwal, D.P., and Goedde, H.W., Life Sci.26 (1980) 1773.PubMedGoogle Scholar
  10. 11.
    Teng, Y.S., Biochem. Genet.19 (1981) 104.Google Scholar
  11. 12.
    Erwin, V.G., and Deitrich, R.A., J. biol. Chem.241 (1966) 3533.PubMedGoogle Scholar
  12. 13.
    Garcin, F., Kasienczuk, D., Radouco-Thomas, S., Côté, J., and Radouco-Thomas, C., Prog. Neuro-Psychopharmac.5 (1981) 619.Google Scholar
  13. 14.
    Garcin, F., Côté, J., Radouco-Thomas, S., Kasienczuk, D., Chawla, S., and Radouco-Thomas, C., Comp. Biochem. Physiol.75B, (1983) 205.Google Scholar
  14. 15.
    Garcin, F., Côté, J., Radouco-Thomas, S., Chawla, S., and Radouco-Thomas, C., Experientia39 (1983) 1122.Google Scholar
  15. 16.
    Garcin, F., Côté, J., Lau You Hin, G. and Chawla, S., Alcoholism/Clin. exp. Res.8 (1984) 91.Google Scholar
  16. 17.
    Liétaert, M.C., Libion-Mannaert, M., Wattiaux-De Conink, S., and Elens, A., Experientia (1984) in press.Google Scholar
  17. 18.
    Lau You Hin, G., Côté, J., and Garcin, F., Proc. ACFAS, Abstr. 3 (1984).Google Scholar
  18. 19.
    Heinstra, P.W., Eiss, K.Th., Schoonen, W.G., Aben, W., de Winter, A.J., Van der Horst, D.J., Van Marrewijk, W.J., Beenakers, A.M., Scharloo, W., and Thörig, G.E., Genetica60 (1983) 129.Google Scholar
  19. 20.
    Sofer, W., and Ursprung, H., J. biol. Chem.243 (1968) 3110.PubMedGoogle Scholar
  20. 21.
    Bradford, M., Analyt. Biochem.72 (1976) 248.PubMedGoogle Scholar
  21. 22.
    Parsons, P.A., Stanley, M., and Spence, G.E., Aust. J. Zool.27 (1979) 747.Google Scholar
  22. 23.
    Hinson, J.A., and Neal, R.A. J. biol. Chem.247 (1972) 7106.PubMedGoogle Scholar
  23. 24.
    Li, T.K., Adv. Enzym.45 (1977) 427.Google Scholar
  24. 25.
    Day, T.H., Hillier, P.C., and Clark, B., Biochem. Genet.11 (1974) 141.PubMedGoogle Scholar
  25. 26.
    Since submission of this manuscript, our in vivo data have shown that the ADH-null mutant strain Adhn4 (which also lacks ALDOX activity) is indeed able to use low acetaldehyde concentrations as food for energy production.Google Scholar

Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • F. Garcin
    • 1
    • 2
  • C. Larochelle
    • 1
    • 2
  • G. Lau You Hin
    • 1
    • 2
  • J. Côté
    • 1
    • 2
  1. 1.Laboratoire de Neuropsychopharmacologie et Neurotoxicologie, Unité de Recherche sur l'Abus des Drogues et de l'Alcool (Unité RADA)Hôpital St-François d'AssiseCanada
  2. 2.Département de Pharmacologie, Faculté de MédecineUniversité LavalCanada

Personalised recommendations