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Titers of ecdysone, 20-hydroxyecdysone and juvenile hormone III throughout the life cycle of a hemimetabolous insect, the ovoviviparous cockroach Nauphoeta cinerea

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Summary

Titers of ecdysone, 20-hydroxyecdysone and juvenile hormone III were measured in whole body extracts or hemolymph of embryos, first, penultimate and last stadium nymphs, and adult females ofNaupoheta cinerea. We used a gas-chromatography/mass spectrometry method for quantifying juvenile hormone and a radio-immunoassay for ecdysteroid determination. Juvenile hormone III is particularly abundant in the embryonic stage (up to 960 ng/g), at a low level in first and penultimate stadium nymphs (2–10 ng/ml) and almost absent in the last nymphal stadium; in the adult female the juvenile hormone titer rises to 180 ng/ml in hemolymph during rapid oocyte growth. The titers of ecdysone and 20-hydroxyecdysone undergo similar fluctuations in the embryonic and nymphal stages, being highest at the time of cuticle formation in the embryo and a few days before the nymphal and adult molts (around 100–200 ng/ml for exdysone and 2–4 μg/ml for 20-hydroxyecdysone).

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Authors and Affiliations

  1. Division of Animal Physiology, University of Bern, Erlachstrasse 9a, CH-3012, Bern, Switzerland

    B. Lanzrein, V. Gentinetta, H. Abegglen, F. C. Baker, C. A. Miller & D. A. Schooley

  2. Dept. of Biochemistry, Zoecon Corporation, 975 California Avenue, 94304, Palo Alto, California, USA

    B. Lanzrein, V. Gentinetta, H. Abegglen, F. C. Baker, C. A. Miller & D. A. Schooley

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  1. B. Lanzrein
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  2. V. Gentinetta
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  3. H. Abegglen
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  4. F. C. Baker
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  5. C. A. Miller
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  6. D. A. Schooley
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Additional information

Acknowledgments. We thank Mrs A. Tschan for rearing the cockroaches, Mr M. Kaltenrieder for drawing the graphs, Mr G.C. Jamieson and Mrs C. Reuter for GC/MS analyses. We are also grateful to the Swiss National Science Foundation (grant no. 3.291-0.82 to B. Lanzrein) and the United States National Science Foundation (grant no. PCM 82-08665 to D.A. Schooley) for their financial support.

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Lanzrein, B., Gentinetta, V., Abegglen, H. et al. Titers of ecdysone, 20-hydroxyecdysone and juvenile hormone III throughout the life cycle of a hemimetabolous insect, the ovoviviparous cockroach Nauphoeta cinerea. Experientia 41, 913–917 (1985). https://doi.org/10.1007/BF01970010

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