Adrenaline-stimulated, aspirin-sensitive synthesis of histamine in the rat
- 16 Downloads
The intravenous injection of 40 μg/kg of adrenaline raised total rat lung histamine from 5.3±0.7 μg, to 8.4±0.7 μg and rat skin histamine from 624±51 μg to 835±85 within 5 min. These changes were no longer apparent after 10 min. Stomach histamine was unaffected. Blood drawn 2 min after the injection of adrenaline failed to show an increased content of histamine. Rats given 1 mg/kg of compound 48/80, had greatly elevated levels of histamine in blood, but exhibited no increase in lung histamine. This result, as well as the extent of the increase of histamine observed in skin, which cannot be accounted for in any other way, point towards stepped-up local synthesis as the origin of the effect of adrenaline. Aspirin (20 mg/kg, intravenously 10 min prior to adrenaline), prevented increases of skin histamine. Evidence suggesting mast cells as the site of action of adrenaline, is discussed.
KeywordsAspirin Mast Cell Histamine Adrenaline Elevate Level
Unable to display preview. Download preview PDF.
- A.M. Rothschild, A. Castania andR.S.B. Cordeiro,Comsumption of Kininogen, Formation of Kinin and Activation of Arginine Ester Hydrolase in Rat Plasma by Rat Peritoneal Fluid Cells in the Presence of 1-Adrenaline. Specific Inhibition by Aspirin, Naunyn-Schmiedebergs Arch. Pharmacol.285, 243–256 (1974).CrossRefPubMedGoogle Scholar
- W. Feldberg andA. Talesnik,Reduction of Tissue Histamine by Compound 48/80, J. Physiol. (Lond.)120, 550–559 (1953).Google Scholar
- M.P. Oliveira Antonio, F. Fernandes andA.M. Rotschild,Increased Lung Histamine Content and Mast Cell Degranulation Evoked by Epinephrine in the Rat. V Intern. Congress Pharmacol. Abstracts Volunt. Papers, p. 8, San Francisco (1972).Google Scholar
- I. Vugman andM. Rocha E. Silva,Biological Determination of Histamine in Living Tissues and Body Fluids, Handb. exp. Pharmacol.18, 97–109 (1966).Google Scholar
- E. Rosengren andS.E. Svensson,Histamine Formation in Rat Gastric Mucosa and Lung after Injecting Reserpine or Adrenaline, Br. J. Pharmac.37, 659–665 (1969).Google Scholar
- P. Graham, P. Kahlson andE. Rosengren,Histamine Formation in Physical Exercise, Anoxia and Under the Influence of Adrenaline and Related Substances, J. Physiol (Lond.)172, 174–188 (1964).Google Scholar
- M. Reichgott,Effects of Endotoxin on Histamine Metabolism in Vivo and in Vitro. VI Intern. Congress Pharmacol. Abstract, p. 235, Helsinki (1975).Google Scholar
- I. Mota, A.G. Ferri andS. Yoneda,The Distribution of Mast Cells in the Digestive Tract of Laboratory Animals: its Bearing on the Problem of the Localization of Histamine in Tissues, Quart. J. micr. Sci.97, 251–257 (1956).Google Scholar
- A.M. Rothschild andM.P.O. Antonio,Histamine Release and Mast Cell Degranulation by Sypathetic Stimulin the Rat. Histamine Symp. Dowden, Hutchinson & Ross pp 81–90. Stroudsburg, Penn. (1973).Google Scholar
- J.C. Gomes, M.Sc. Dissertation, p. 33, School of Medicine of Ribeirão Preto (1976)Google Scholar
- A.M. Rothschild, E. Tamburus andJ.F. Fracasso,Activation of Cellular Prokinogenase by Adrenaline or Carbamylcholine: Biochemical Aspects. Intern. Symp. on Inflammation and Antiinflammatory Therapy. Soc. Bras. Farmacol. Terap. Exp. Abstracts, p. 44, Rio de Janeiro (1976).Google Scholar