The present study aims specifically at obtaining a comparison of the acute toxicity of cypermethrin (CY), a type I pyrethroid, and permethrin (PERM), a type II pyrethroid, administered orally as a single dose to neonatal and adult rats, and at assessing the importance of pyrethroid biotransformation in CY and PERM toxicity through use of drug metabolism inhibitors. Our experiments show that CY is more toxic than PERM to adult and neonatal rats. The sensitivity of neonatal rats both to CY and to PERM toxicity is higher, the younger the animals. CY is much more toxic than PERM in the neonatal rat, compared with the adult. In rats aged 8, 16, and 21 days, pretreatment with piperonil butoxide (PB), a monooxygenase inhibitor, or with tri-o-tolyl phosphate (TOTP), an esterase inhibitor, does not produce significant variations in the lethal effects of CY and PERM. Instead, in the adult rats, a significant increase in CY (X2=5.97;p<0.05) and PERM (X2=4.37;p<0.05) mortality occurred in rats pretreated with esterase inhibitors, whereas no increase in CY and PERM toxicity was found in adult animals pretreated with monooxygenase inhibitor. It was concluded that the higher level of sensitivity of the neonate rat to pyrethroid toxicity is probably due to incomplete development of the enzymes which catalyze the metabolism of pyrethroids in the liver of young animals. It is suggested that ester hydrolysis is an important pyrethroids detoxification reaction in the adult rat.
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Abernathy CO, Ueda K, Engel JL, LC Gaughan JE, Casida JE (1973) Substrate specificity and toxicological significance of pyrethroid-hydrolyzing esterase of mouse liver microsomes. Biochem Physiol 3: 300–311
Cohen SD, Murphy SD (1970) Comparative potentiation of malathion by triorthotolylphosphate in four classes of vertebrate. Toxicol Appl Pharmacol 16: 88–95
Edwards RP, Millburn DH, Huston P (1986) Comparative toxicity of cis-cypermethryn in rainbow trout, frog, mouse, and quail. Toxicol Appl Pharmacol 84: 512–522
Elliott M (1977) Synthetic insecticides designed from natural pyrethrins. Pontif Acad Sci Scr Varia 41: 57–184
Elliott M, Farnham MAW, Janes NF, Needham PH, Pearson BC (1967) 5-Benzyl-3-furyl-methyl chrysanthemate. A new potent insecticide. Nature 213: 493–494
Eriksson P, Fredriksson (1991) Neurotoxic effects of two pyrethroids, bioallethrin and deltamethrin, on immature and adult mice: changes in behavioral and muscarinic receptor variables. Toxicol Appl Pharmacol 108: 78–85
Eriksson P, Nordberg A (1990) Effects of two pyrethroids, bioallethrin and deltamethrin, on subpopulations of muscarinic and nicotinic receptors in the neonatal mouse brain. Toxicol Appl Pharmacol 102: 456–463
Fisher RH, Yates F (1948) Statistical tables for agricultural, biological and medical research. Oliver and Boyd, Edinburgh
Fouts JR, Adamson RH (1959) Drug metabolism in the newborn rabbit. Science 129: 897–898
Gaughan LC, Unai T, Casida JE (1977) Permethrin metabolism in rats. Agric Food Chem 25: 9–17
Gaughan LC, Ackerman ME, Unai T, Casida JE (1978) Distribution and metabolism of trans- and cis-permethrin in lactating Jersey cows. J Agric Food Chem 26: 613–618
Glickman AH, Lech JJ (1982) Differential toxicity of trans-permethrin in rainbow trout and mice. II. Role of target organ sensitivity. Toxicol Appl Pharmacol 66: 162–171
Glickman AH, Weitman SD, Lech JJ (1982) Differential toxicity of trans-permethrin in rainbow trout and mice. I. Role of biotransformation. Toxicol Appl Pharmacol 66: 153–161
Hutson DH, Millburn P (1991) Enzyme-mediated selectivity of an organophosphate and a pyrethroid: some examples from a range of animals. Biochem Soc Trans 19: 737–740
Kavlock R, Chernoff N, Baron R, Linder R, Rogers E, Carver B (1979) Toxicity studies with decamethrin, a synthetic insecticide. J Environ Pathol Toxicol 2: 751–765
Lawrence LJ, Casida JE (1982) Pyrethroid toxicology: mouse intracerebral structure-toxicity relationships. Pestic Biochem Physiol 18: 9–14
Miyamoto J (1976) Degradation, metabolism and toxicity of synthetic pyrethroyds. Environ Health Perspect 14: 15–28
Ray DE, Cremer JE (1979) The action of decamethrin (a synthetic pyrethroid) on the rat. Pestic Biochem Physiol 10: 333–340
Ruzo LO, Engel JL, Casida JE (1979) Decamethrin metabolites from oxidative hydrolytic and conjugative reactions in mice. J Agric Food Chem 27: 725–731
Soderlund DM, Casida JE (1977) Effects of pyrethroid structure on rates of hydrolysis and oxidation by mouse liver microsomal enzymes. Pestic Biochem Physiol 7: 391–401
Suzuki T, Miyamoto J (1978) Purification and properties of pyrethroid carboxyesterase in rat liver microsome. Pestic Biochem Physiol 8: 186–198
Thompson WR, Weil CS (1952) On the construction of tables for movings average interpolation. Biometrics 8: 51–54
Verschoyle RD, Aldridge WN (1980) Structure-activity relationships of some pyrethroids in rats. Arch Toxicol 45: 325–329
Verschoyle RD, Barnes JM (1972) Toxicity of natural and synthetic pyrethrins to rats. Pestic Biochem Physiol 2: 308–311
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Cantalamessa, F. Acute toxicity of two pyrethroids, permethrin, and cypermethrin in neonatal and adult rats. Arch Toxicol 67, 510–513 (1993). https://doi.org/10.1007/BF01969923
- Acute toxicity
- Drug metabolism inhibitors