Transgenic Research

, Volume 5, Issue 3, pp 209–211 | Cite as

Genomic DNA analysis from mouse toe lysates

  • Nancy L. Nadon
  • Karen Draeger
Technical Note

Abstract

The protocol described in this report provides a simple, accurate and efficient assay for detection of transgenes and mutations in large colonies of rodents, using crude lysates prepared from the digit cut from the animals for identification purposes. This can be done as early as 6 days of age, minimizing trauma to the mice and allowing assays to be completed long before weaning.

Key words

transgene PCR mutation 

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References

  1. Higuchi, R. (1989) Simple and rapid preparation of samples of PCR. In H.A. Erlich, ed.,PCR Technology, pp. 31–8. New York: Stockton Press.Google Scholar
  2. Hogan, B., Beddington, R., Costantini, F. and Lacy, E. (1994)Manipulating the Mouse Embryo, 2nd edition New York: Cold Spring Harbor Laboratory Press.Google Scholar
  3. Nadon, N.L., Arnheiter, H. and Hudson, L.D. (1994) A combination of PLP and DM20 transgenes promotes partial myelination in the jimpy mouse.J. Neurochem. 63, 822–33.PubMedGoogle Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Nancy L. Nadon
    • 1
  • Karen Draeger
    • 1
  1. 1.Department of Biological Sciences and Oklahoma Center for Molecular MedicineUniversity of TulsaTulsaUSA

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