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Evaluation of two methods for rapid testing for beta-lactamase production inBacteroides andFusobacterium

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Abstract

A total of 978 strains ofBacteroides andFusobacterium were tested for beta-lactamase production by a disk test (Cefinase) and a microtiter nitrocefin assay. In 83 % of strains both tests were positive and in 14.8 % both were negative. In 1.7 % of strains the disk test was positive and the microtiter test negative, and in 0.4 % the disk test negative but the microtiter test positive. The disk test was less discriminatory in detecting amoxicillin-resistant strains. The microtiter test was less sensitive than the disk test, but more discriminatory if results were read within 1 h forFusobacterium spp., within 8 h for theBacteroides fragilis group, and within 2 h for otherBacteroides spp. Neither test should be used clinically at present.

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Authors and Affiliations

  1. Department of Pathology (Clinical Microbiology), Hershey Medical Center, PO Box 850, 17033, Hershey, Pennsylvania, USA

    P. C. Appelbaum & S. K. Spangler

  2. Case Western Reserve University and University Hospitals of Cleveland, 2047 Abington Rd., 44106, Cleveland, Ohio, USA

    M. R. Jacobs

Authors
  1. P. C. Appelbaum
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  2. S. K. Spangler
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  3. M. R. Jacobs
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Appelbaum, P.C., Spangler, S.K. & Jacobs, M.R. Evaluation of two methods for rapid testing for beta-lactamase production inBacteroides andFusobacterium . Eur. J. Clin. Microbiol. Infect. Dis. 9, 47–50 (1990). https://doi.org/10.1007/BF01969535

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