An insertional mutation in theBTF3 transcription factor gene leads to an early postimplantation lethality in mice
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The gene that encodes the general transcription factor known asBTF3 was disrupted in mouse embryonic stem cells in a random mutagenesis screen for developmental genes with the ROSA β-geo (Friedrich and Soriano, 1991) retroviral gene trap vector. TheBTF3 mutation was transmitted through the germline of chimaeric mice. While the endogenousBTF3 gene is ubiquitously expressed, the expression pattern of the β-galactosidase reporter gene present in the gene trap vector inBTF3 heterozygotes was restricted. Mice homozygous for the mutant allele died soon after implantation, around embryonic day 6.5. Thus,BTF3 is essential for postimplantation development. The isolation of theBTF3 sequences in this ROSA β-geo insertion was facilitated by a relatively simple singlelacZ primer reverse transcription PCR strategy.
Keywordsretrovirus gene traps embryonic stem cells mutagenesis BTF3
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