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, Volume 25, Issue 1–2, pp 171–181 | Cite as

Proteoglycan biosynthesis by rabbit articular chondrocytes treated withd-penicillamine

  • P. Legendre
  • M. Bouakka
  • M. Langris
  • J. -P. Pujol
  • R. Beliard
  • G. Loyau
  • J. Bocquet
Immunosuppression and Inflammations


Rabbit articular chondrocytes in confluent monolayer cultures were treated withd-Penicillamine (d-Pen) during 3 or 5 days. The [35S]-sulfate incorporation in neosynthesized proteoglycans was not modified byd-Pen doses ranging from 50 to 800 μg/ml. After treatment during 5 days withd-Pen concentrations of 50 or 400 μg/ml, the chemical characteristics of proteoglycans from medium and cell-layer were determined. The aggregation capacity of proteoglycans from medium, the monomer molecular size, the glycosaminoglycan chain length and the relative rates of the different glycosaminoglycans (chondroitins, chondroitin 6-sulfate, chondroitin 4-sulfate, hyaluronic acid) remained unchanged. These results suggest thatd-Pen does not alter some of the cartilage mechanical properties due to the presence of proteoglycans.


Mechanical Property Chain Length Hyaluronic Acid Chemical Characteristic Molecular Size 
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Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • P. Legendre
    • 1
  • M. Bouakka
    • 1
  • M. Langris
    • 1
  • J. -P. Pujol
    • 1
  • R. Beliard
    • 2
  • G. Loyau
    • 1
  • J. Bocquet
    • 1
  1. 1.Laboratoire de Biochimie du Tissu Conjonctif, U.F.R. de SciencesC.H.U. Côte de NacreCaen cedexFrance
  2. 2.Laboratoire d'Anatomie Pathologique, U.F.R. de MédecineC.H.U. Côte de NacreCaen cedexFrance

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