Advertisement

Agents and Actions

, Volume 25, Issue 1–2, pp 106–114 | Cite as

Anti-pokeweed mitogen antiserum inhibits and enhances blastogenesis of mononuclear cells induced by pokeweed mitogen

  • H. G. Uiterdijk
  • E. de Vries
  • J. P. van der Weij
  • H. J. Kornman
  • C. J. P. van der Veen
  • A. Cats
Immunosuppression and Inflammations

Abstract

The interaction between pokeweed mitogen (PWM) and peripheral blood mononuclear cells (PBMC) was investigated using rabbit anti-PWM antiserum (anti-PWM) and125 I-PWM. Incubation of PBMC with PWM in the presence of anti-PWM resulted in an inhibition of the mitogenic effect of PWM. Anti-PWM predominantly blocked the interaction of PWM with monocytes, which is essential for optimal stimulation of lymphoid cells with PWM.

Addition of anti-PWM to PBMC at several time-points after incubation with PWM showed inhibition of mitogenic activity when anti-PWM was added within 8 hours. However, enhancement of PWM-induced blast cell formation was found when anti-PWM was added after 48 hours. Further analysis revealed that the inhibition of PWM stimulation was mediated by the F(ab′)2 part of anti-PWM IgG. On the other hand F(ab′)2-anti-PWM was not able to enhance the effect of PWM.

Incubation of PBMC with125I-PWM and anti-PWM simultaneously, decreased the binding of PWM to both lymphocytes and monocytes. In contrast, addition of anti-PWM 48 hours after the incubation of PBMC with PWM resulted in an increased binding of PWM to monocytes.

These results show that anti-PWM can moculate the lymphocyte reaction to PWM and suggest two possible mechanisms by which PWM can stimulate PBMC, both of which are dependent on the interaction of PWM with monocytes.

Keywords

Mononuclear Cell Peripheral Blood Mononuclear Cell Lymphoid Cell Cell Formation Blast Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    T. A. Waldmann and S. Broder,Polyclonal B-cell activators in the study of the regulation of immunoglobulin synthesis in the human system. InAdvances in Immunology, vol. 32. (Ed. F. J. Dixon and H. G. Kunkel) pp. 1–63. Academic Press, N. Y. 1982.Google Scholar
  2. [2]
    E. de Vries, G. J. M. Lafeber, J. P. van der Weij, A. C. van Buijsen, P. C. J. Leijh, and A. Cats,Pokeweed-mitogen induced lymphocyte proliferation: the effect of stimulation on mononuclear phagocytic cells. Immunology40, 177–182 (1990).Google Scholar
  3. [3]
    K. L. Banks and I. C. McGuire,Regulation by antibody of phytolectin induced lymphocyte proliferation. I. Evidence for two mechanisms of supperssion, J. Immunol.114, 1307–1313 (1975).PubMedGoogle Scholar
  4. [4]
    S. A. Rosenberg and P. E. Lipskey,Monocyte dependence of pokeweed mitogen-induced differentiation of immunoglobulin-secreting cells from human peripheral blood mononuclear cells. J. Immunol.122, 926–931 (1979).PubMedGoogle Scholar
  5. [5]
    H. C. Stevenson, P. J. Miller, M. J. Waxdal, B. F. Haynes, C. A. Thomas and A. S. Fauci,Interaction of pokeweed mitogen with monocytes in the activation of human lymphocytes, Immunology49, 633–640 (1983).PubMedGoogle Scholar
  6. [6]
    D. L. Thiele and P. E. Lipsky,The accessory function of phagocytic cells in human T cell and B cell responses. J. Immunol.129, 1033–1040 (1982).PubMedGoogle Scholar
  7. [7]
    J. E. de Vries, A. P. Caviles, W. S. Bont and J. Mendelsohn,The role of monocytes in human lymphocyte activation by mitogens. J. Immunol.122, 1099–1106 (1979).PubMedGoogle Scholar
  8. [8]
    A. Böyum,Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest.21 (suppl. 97), 77–87 (1968).PubMedGoogle Scholar
  9. [9]
    L. L. Yam, C. Y. Li and W. H. Crosby,Cytochemical identification of monocytes and granulocytes. Am. J. Clin. Pathol.55, 283–290 (1971).PubMedGoogle Scholar
  10. [10]
    J. W. M. van der Meer, J. S. van der Gevel, I. Elzenga-Claassen and R. van Furth,Suspension cultures of mononuclear phagocytes in the teflon culture bag. Cell. Immunol.42, 208–212 (1979).CrossRefPubMedGoogle Scholar
  11. [11]
    E. de Vries, J. P. van der Weij, C. J. P. van der Veen and A. Cats,Characterization and classification of lymphoid cells after pokeweed mitogen stimulation. Virchows Archives B (Cell. Pathol.)43, 17–30 (1983).Google Scholar
  12. [12]
    C. H. Wouters, J. P. van der Weij, G. J. M. Koper, W. Th. Daems, E. de Vries and J. S. Ploem,Measurement of singlecell DNA synthesis by pokeweed mitogen-stimulated mononuclear cells with combined light and scanning electron microscopy. Immunology58, 79–85 (1986).PubMedGoogle Scholar
  13. [13]
    A. Voller, D. E. Bidwell and A. Barlett,Enzyme immunoassays in diagnostic medicine. Bulletin WHO53 55–65 (1976).Google Scholar
  14. [14]
    J. I. Thorell and I. Larsson,Lactoperoxidase coupled to polyacrylamide for radio-iodination of proteins to high specific activity. Immunochem.2, 203–206 (1974).CrossRefGoogle Scholar
  15. [15]
    T. Irimura and G. L. Nicolson,Interaction of pokeweed mitogen with poly-(n-Acetyllactosamine)-type carbohydrate chains. Carbohydrate Res.120, 187–195 (1983).CrossRefGoogle Scholar
  16. [16]
    K. Yokoyama, T. Terao and T. Osawa,Carbohydrate-binding specificity of pokeweed mitogens. Biochem. Biophys. Acta.538, 384–396 (1978).PubMedGoogle Scholar
  17. [17]
    T. Kina, S. Nishikawa and Y. Katsura,T-cell regulation of pokeweed-mitogen-induced polyclonal immunoglobulin production in mice. Immunology46, 538–587 (1982).Google Scholar
  18. [18]
    J. Pryjama, H.-D. Flad, M. Ernst and A. Pituch-Noworolska,Pokeweed mitogen activated suppressor T-cells preferentially reduce immunoglobulin secretion by differentiated B-lymphocytes. Immunol. Lett.13, 273–279 (1986).CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • H. G. Uiterdijk
    • 1
  • E. de Vries
    • 1
  • J. P. van der Weij
    • 1
  • H. J. Kornman
    • 2
  • C. J. P. van der Veen
    • 1
  • A. Cats
    • 1
  1. 1.Department of RheumatologyUniversity HospitalLeidenThe Netherlands
  2. 2.Institute of EpidemiologyErasmus UniversityRotterdamThe Netherlands

Personalised recommendations