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Transgenic Research

, Volume 5, Issue 5, pp 359–362 | Cite as

Environmental risk assessment of releases of transgenic plants containing virus-derived inserts

  • David J. Robinson
Letter to the Editor

Abstract

Sequences derived from the genomes of plant viruses are being used to provide virus resistance in transgenic crop plants. Although the environmental hazards associated with the release of such plants have been discussed widely, it has not been possible to reach generally acceptable conclusions about their safety. A case-by-case approach to the risk assessment of real examples is recommended as a means of building up confidence and of indicating areas of uncertainty. A logical framework for risk assessment is suggested, a key feature of which is identification of the viruses in the release environment that may infect the transgenic plants. Each of these is considered in relation to each of the three main classes of hazard (transcapsidation, recombination and synergism), and the risk associated with each event is analysed.

Keywords

virus resistant plants virus-derived inserts transcapsidation recombination synergism environmental risk assessment 

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References

  1. Anon. (1995) Transgenic virus-resistant plants and new plant viruses. Proceedings of an American Institute of Biological Sciences Workshop, Beltsville, MD.Google Scholar
  2. Candelier-Harvey, P. and Hull, R. (1993) Cucumber mosaic virus genome is encapsidated in alfalfa mosaic virus coat protein expressed in transgenic tobacco plants.Transgenic Res. 2, 277–85.Google Scholar
  3. Cooper, B., Lapidot, M., Heick, J.A., Dodds, J.A. and Beachy, R.N. (1995) A defective movement protein of TMV in transgenic plants confers resistance to multiple viruses whereas the functional analog increases susceptibility.Virology 206, 307–13.PubMedGoogle Scholar
  4. Cooper, J.I., Edwards, M.L., Rosenwasser, O. and Scott, N.W. (1994) Transgenic resistance genes from nepoviruses: efficacy and other properties.N. Z. J. Crop Hort. Sci. 22, 129–37.Google Scholar
  5. de Zoeten, G.A. (1991) Risk assessment: do we let history repeat itself?Phytopathol. 81, 585–6.Google Scholar
  6. Farinelli, L., Malnoë, P. and Collet, G.F. (1992) Heterologous encapsidation of potato virus Y strain O (PVYo) with the transgenic coat protein of PVY strain N (PVYN) inSolanum tuberosum cv. Bintje.Bio/Technology 10, 1020–5.Google Scholar
  7. Gal, S., Pisan, B., Hohn, T., Grimsley, N. and Hohn, B. (1992) Agroinfection of transgenic plants leads to viable cauliflower mosaic virus by intermolecular recombination.Virology 187, 525–33.PubMedGoogle Scholar
  8. Golemboski, D.B., Lomonossoff, G.P. and Zaitlin, M. (1990) Plants transformed with a tobacco mosaic virus nonstructural gene sequence are resistant to the virus.Proc. Natl Acad. Sci. USA 87, 6311–5.PubMedGoogle Scholar
  9. Greene, A.E. and Allison, R.F. (1994) Recombination between viral RNA and transgenic plant transcripts.Science 263, 1423–5.PubMedGoogle Scholar
  10. Hamilton, R.I. (1980) Defenses triggered by previous invaders: viruses. In: Horsfall J.G. and Cowling, E.B. eds.Plant Disease: An Advanced Treatise, pp. 279–303, New York, Academic Press.Google Scholar
  11. Harrison, B.D., Mayo, M.A. and Baulcombe, D.C. (1987) Virusresistance in plants that express cucumber mosaic-virus satellite RNA.Nature 328, 799–802.Google Scholar
  12. Lapidot, M., Gafny, R., Ding, B., Wolf, S., Lucas, W.J. and Beachy, R.N. (1993) A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plants.Plant J. 4, 959–970.Google Scholar
  13. Lecoq, H., Ravelonandro, M., Wipf-Scheibel, C., Monsion, M., Raccah, B. and Dunez, J. (1993) Aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic potyvirus from transgenic plants expressing the capsid protein of plum pox potyvirus.Mol. Plant-Microbe Interactions 6, 403–6.Google Scholar
  14. Lindbo, J.A. and Dougherty, W.G. (1992) Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts.Virology 189, 725–33.Google Scholar
  15. Lommel, S.A. and Xiong, Z. (1991) Reconstitution of a functional red clover necrotic mosaic virus by recombinational rescue of the cell-to-cell movement gene expressed in a transgenic plant.J. Cell. Biochem. 15A, 151.Google Scholar
  16. Lung, M.C.Y. and Pirone, T.P. (1974) Acquisition factor required for aphid transmission of purified cauliflower mosaic virus.Virology 60, 260–4.PubMedGoogle Scholar
  17. Maiti, I.B., Murphy, J.F., Shaw, J.G. and Hunt, A.G. (1993) Plants that express a potyvirus proteinase gene are resistant to virus infection.Proc. natl Acad. Sci. USA 90, 6110–4.PubMedGoogle Scholar
  18. Martin, R.R., Keese, P.K., Young, M.J., Waterhouse, P.M. and Gerlach, W.L. (1990) Evolution and molecular biology of luteoviruses.Ann. Rev. Phytopath. 28, 341–63.Google Scholar
  19. Osbourn, J.K., Sarkar, S. and Wilson, T.M.A. (1990) Complementation of coat protein-defective TMV mutants in transgenic tobacco plants expressing TMV coat protein.Virology 179, 921–5.PubMedGoogle Scholar
  20. Powell Abel, P., Nelson, R.S., De, B., Hoffman, N., Rogers, S.G., Fraley, R.T. and Beachy, R.N. (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.Science 232, 738–43.PubMedGoogle Scholar
  21. Taliansky, M.E. and Garcia-Arenal, F. (1995) Role of cucumovirus capsid protein in long-distance movement within the infected plant.J. Virol. 69, 916–22.PubMedGoogle Scholar
  22. Tamada, T. and Abe, H. (1989) Evidence that beet necrotic yellow vein virus RNa-4 is essential for efficient transmission by the fungusPolymyxa betae.J. Gen. Virol. 70, 3391–8.Google Scholar
  23. Tamada, T. and Kusume, T. (1991) Evidence that the 75k readthrough protein of beet necrotic yellow vein virus RNA-2 is essential for transmission by the fungusPolymyxa betae.J. Gen. Virol. 72, 1497–504.PubMedGoogle Scholar
  24. Tepfer, M. (1993) Viral genes and transgenic plants. What are the potential environmental risks?Bio/Technology 11, 1125–32.Google Scholar
  25. Thornbury, D.W., Patterson, C.A., Dessens, J.T. and Pirone, T.P. (1990) Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C.Virology 178, 573–8.PubMedGoogle Scholar
  26. Valkonen, J.P.T. (1992) Accumulation of potato virus-Y is enhanced inSolanum brevidens also infected with tobacco mosaic virus or potato spindle tuber viroid.Ann. Appl. Biol. 121, 321–7.Google Scholar
  27. Vance, V.B., Berger, P.H., Carrington, J.C., Hunt, A.G. and Shi, X.M. (1995) 5′ proximal potyviral sequences mediate potato virus X/potyviral synergistic disease in transgenic tobacco.Virology 206, 583–90.PubMedGoogle Scholar
  28. Murashige, T. and Skoog, F. (1962) A revised method for rapid growth and bioassays with tobacco tissue cultures.Physiol. Plant. 15, 473–97.Google Scholar
  29. Murray, M.G. and Thompson, W.P. (1980) Rapid isolation of high molecular weight plant DNA.Nucl. Acids Res. 8, 4321–5.PubMedGoogle Scholar
  30. Robbins, M., Carron, T.R. and Webb, K.J. (1993) Detecting transgenes inLotus corniculatus using the polymerase chain reaction.Lotus Newsletter.24, 45–48.Google Scholar
  31. Therrien, M.C. and Grant, W.F. (1982) Induction of two mutants in birdsfoot trefoil (Lotus corniculatus) by X-rays and chemical mutagens.Can. J. Plant Sci. 62, 957–63.Google Scholar
  32. Ulian, E. C., Smith, R.H., Gould, J.H., and McKnight, T.D. (1988) Transformation of plants via the shoot apex.In Vitro Cell Dev. Biol. 21, 951–4.Google Scholar
  33. Ulian, E.C., Magill, J.M. and Smith, R.H. (1994) Expression and inheritance pattern of two foreign genes in petunia.Theor. Appl. Genet. 88, 433–40.Google Scholar
  34. Visser, R.G.F., Hesseling-Meinders, A., Jacobsen, E., Nijdam, H., Witholt, B. and Feenstra, W.J. (1989) Expression and inheritance of inserted markers in binary vector carryingAgrobacterium rhizogenes transformed potato (Solanum tuberosum L.).Theor. Appl. Genet. 78, 705–714.Google Scholar
  35. Wang, Z.-Y., Takamizo, T., Iglesias, V.A., Osusky, M., Nagel, J., Potrykus, I. and Spandenburg, G. (1992) Transgenic plants of tall fescue (Festuca arundinacea Schreb.) obtained by direct gene transfer to protoplasts.Bio/Technology 10, 691–9.PubMedGoogle Scholar
  36. Webb, K.J., Fay, M.F. and Dale, P.J. (1987) An investigation of morphogenesis within the genusTrifolium.Plant Cell Tissue Organ Cult. 11, 37–46.Google Scholar
  37. Webb, K.J., Jones, S., Robbins, M.P. and Minchin, F.R. (1990) Characterization of transgenic root cultures ofTrifolium repens, T. pratense andLotus corniculatus and transgenic plants ofL. corniculatus.Plant Sci. 70, 243–254.Google Scholar
  38. Webb, K.J., Robbins, M.P. and Mizen, S. (1994a) Expression of GUS in primary transformants and segregation patterns of GUS, TL-and TR-DNA in the T1 generation of hairy root transformants ofLotus corniculatus.Transgenic Res. 3, 232–240.Google Scholar
  39. Webb, K.J., Mizen, S. and Cooke, D.E. (1994b) A long term study of GUS activity in hairy root cultures and primary transformants inLotus corniculatus.Lotus Newsletter. 25, 28–30.Google Scholar
  40. Yu, J.P. and Shao, Q.Q. (1991) Transformation ofLotus corniculatus L. mediated byAgrobacterium tumefaciens.Sci. China 34, 932–7.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • David J. Robinson
    • 1
  1. 1.Scottish Crop Research InstituteDundeeUK

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