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, Volume 22, Issue 1–2, pp 75–81 | Cite as

Apamin and nonadrenergic inhibition of guinea pig trachealis

  • M. E. Zacour
  • B. Collier
  • J. G. Martin
Allergy

Abstract

Apamin has been shown to antagonize the nonadrenergic, noncholinergic (NANC) inhibitory system in guinea pig taenia coli. We have examined the effects of apamin on the nonadrenergic noncholinergic inhibitory system and its putative transmitters in isolated guinea pig trachea. Electrical field stimulation (ES) of isolated trachea pretreated with atropine and propranolol evoked reproducible relaxations that were blocked by tetrodoxin, but were unaffected by apamin. Vasoactive intestinal peptide (VIP), adenosine (AD), and adenosine triphosphate (ATP) produced concentration-dependent inhibition of histamine (H)-induced contractions of isolated trachea but the inhibitory actions of these agents were not significantly affected by apamin. In contrast, apamin virtually abolished ES-evoked relaxations in guinea pig isolated taenia caeci, and reduced the inhibition of H-induced contraction by ATP from 40% to 1%. We conclude that neither the NANC inhibitory system in the guinea pig trachea nor its putative mediators VIP, AD, and ATP are antagonized by apamin, in contrast to taenia caeci.

Keywords

Adenosine Histamine Propranolol Triphosphate Atropine 

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References

  1. [1]
    B. Banks, C. Brown, G. M. Burgess, G. Burnstock, M. Claret, T. M. Cocks and D. H. Jenkinson,Apamin blocks certain neurotransmitter-induced increases in potassium permeability. Nature282, 415–417 (1979).PubMedGoogle Scholar
  2. [2]
    C. Brown and G. Burnstock,Evidence in support of the P1/P2 purinoceptor hypothesis in the guinea pig taenia coli. Br. J. Pharmac.73, 617–624 (1981).Google Scholar
  3. [3]
    E. Bulbring and T. Tomita,Properties of the inhibitory potential of smooth muscle as observed in response to field stimulation of the guinea pig taenia coli. J. Physiol. (London)189, 299–316 (1967).Google Scholar
  4. [4]
    G. Burnstock,Purinergic nerves. Pharmac. Rev.24, 509–581 (1972).Google Scholar
  5. [5]
    G. Burnstock, T. Cocks, L. Kasakov and H. K. Wong,Direct evidence for ATP release from non-adrenergic, noncholinergic (“purinergic”) nerves in the guinea pig taenia coli and bladder. Eur. J. Pharmacol.49, 145–149 (1978).CrossRefPubMedGoogle Scholar
  6. [6]
    A. C. Cameron, C. F. Johnson, C. T. Kirkpatrick and M. C. A. Kirkpatrick,The quest for the inhibitory neurotransmitter in bovine tracheal smooth muscle. Q. J. Exp. Physiol.68, 413–426 (1983).PubMedGoogle Scholar
  7. [7]
    R. Casteels,Membrane potential in smooth muscle cells. InSmooth Muscle: An Assessment of Current Knowledge (Eds. E. Bulbring, A. F. Brading, A. W. Jones and T. Tomita), Edward Arnold Publishers Ltd, London 1981.Google Scholar
  8. [8]
    R. F. Coburn and T. Tomita,Evidence for nonadrenergic inhibitory nerves in the guinea pig trachealis muscle. Am. J. Physiol.224, 1072–1080 (1973).PubMedGoogle Scholar
  9. [9]
    R. A. Coleman,Effects of some purine derivatives on the guinea pig trachea and their interaction with drugs that block adenosine uptake. Br. J. Pharmacol.57, 51–57 (1976).PubMedGoogle Scholar
  10. [10]
    R. A. Coleman,Purine antagonists in the identification of adenosine-receptors in guinea pig trachea and the role of purines in nonadrenergic inhibitory neurotransmission. Br. J. Pharmacol.69, 359–366 (1980).PubMedGoogle Scholar
  11. [11]
    E. E. Daniel, A. Helmy-Elkholy, L. P. Jager and M. S. Kannan,Neither a purine nor VIP is the mediator of inhibitory nerves of opossum oesophageal smooth muscle. J. Physiol.336, 243–260 (1983).PubMedGoogle Scholar
  12. [12]
    J. S. Davison, M. Al-Hassani, R. Crowe and G. Burnstock,The non-adrenergic, inhibitory innervation of the guinea pig gallbladder. Pflugers Arch.377, 43–49 (1978).CrossRefPubMedGoogle Scholar
  13. [13]
    J. S. Douglas, C. Brink and A. Bouhuys,Actions of histamine and other drugs on guinea pig airway smooth muscle. InLung Cells in Disease (Ed. A. Bouhuys), Elsevier/North Holland Biomedical Press, 1976.Google Scholar
  14. [14]
    R. Frew and P. M. Lundy,Evidence against ATP being the nonadrenergic, noncholinergic inhibitory transmitter in guinea pig stomach. Eur. J. Pharmacol.81, 333–336 (1982).CrossRefPubMedGoogle Scholar
  15. [15]
    J. Hills, L. A. Meldrum, P. Klarskov and G. Burnstock,A novel non-adrenergic, non-cholinergic nerve-mediated relaxation of the pig bladder neck: an examination of possible neurotransmitter candidates. Eur. J. Pharmacol.99, 287–293 (1984).CrossRefPubMedGoogle Scholar
  16. [16]
    G. K. Hogaboom, J. P. O'Donnell and J. S. Fedan,Purinergic receptors: photoaffinity analog of adenosine triphosphate is a specific adenosine triphosphate antagonist. Science208, 1273–1275 (1980).PubMedGoogle Scholar
  17. [17]
    S. Holmgren,The effects of putative non-adrenergic, noncholinergic autonomic transmitters on isolated strips from the stomach of the rainbow trout, salmo gairdneri. Comp. Biochem. Physiol.74, 229–238 (1983).CrossRefGoogle Scholar
  18. [18]
    C. Irvin, R. Martin and P. T. Macklem,Non-adrenergic inhibitory nerves. Eur. J. Respir. Dis.64, 133–137 (1983).Google Scholar
  19. [19]
    Y. Ito and K. Takeda,Non-adrenergic inhibitory nerves and putative transmitters in the smooth muscle of cat trachea. J. Physiol.330, 497–511 (1982).PubMedGoogle Scholar
  20. [20]
    L. P. Jager,The effect of catecholamines and ATP on the smooth muscle cell membrane of the guinea pig taenia coli. Eur. J. Pharmacol.25, 372–382 (1974).CrossRefPubMedGoogle Scholar
  21. [21]
    D. H. Jenkinson,Peripheral actions of apamin. TIPS reviews Dec, 318–320 (1981).Google Scholar
  22. [22]
    M. Jodal, O. Lundgren and A. Sjoqvist,The effect of apamin on non-adrenergic, non-cholinergic vasodilator mechanisms in the intestines of the cat. J. Physiol.338, 207–219 (1983).PubMedGoogle Scholar
  23. [23]
    C. T. Kirkpatrick,Tracheobronchial smooth muscle. InSmooth Muscle: An Assessment of Current Knowledge (Eds. E. Bulbring, A. F. Brading, A. W. Jones, T. Tomita). Edward Arnold Publishers Ltd, London 1981.Google Scholar
  24. [24]
    H. Kuriyama, T. Osa and N. Toida,Nervous factors influencing themembrane activity of intestinal smooth muscle. J. Physiol. (London)191, 257–270 (1967).Google Scholar
  25. [25]
    A. J. Maas,The effect of apamin on responses evoked by field stimulation in guinea pig taenia caeci. Eur. J. Pharmacol.73, 1–9 (1981).CrossRefPubMedGoogle Scholar
  26. [26]
    A. J. Maas and A. Den Hertog,The effect of apamin on the smooth muscle cells of the guinea pig taenia coli. Eur. J. Pharmacol.58, 151–156 (1979).CrossRefPubMedGoogle Scholar
  27. [27]
    I. MacKenzie and G. Burnstock,Evidence against vasoactive intestinal polypeptide being the non-adrenergic, noncholinergic inhibitory transmitter released from nerves supplying the smooth muscle of the guinea pig taenia coli. Eur. J. Pharmacol.67, 255–264 (1980).CrossRefPubMedGoogle Scholar
  28. [28]
    Y. Matsuzaki, Y. Hamasaki and S. Said,Vasoactive intestinal peptide: a possible transmitter of nonadrenergic relaxation of guinea pig airways. Science210, 1252–1253 (1980).PubMedGoogle Scholar
  29. [29]
    M. J. Muller and H. P. Baer,Apamin, a nonspecific antagonist of smooth muscle relaxants. Naunyn Schmiedeberg's Arch. Pharmacol.311, 105–107 (1980).CrossRefGoogle Scholar
  30. [30]
    J. B. Richardson and T. Bouchard,Demonstration of a nonadrenergic inhibitory nervous system in the trachea of the guinea pig. J. Allergy Clin. Immunol.56, 473–480 (1975).CrossRefPubMedGoogle Scholar
  31. [31]
    D. G. Satchell,Nucleotide pyrophosphatase antagonizes responses to adenosine 5-triphosphate and non-adrenergic noncholinergic inhibitory nerve stimulation in the guinea pig isolated taenia coli. Br. J. Pharmacol.74, 319–321 (1981).PubMedGoogle Scholar
  32. [32]
    M. F. Shuba and I. A. Vladimirova,Effect of apamin on the electrical responses of smooth muscle to adenosine 5′-triphosphate and to non-adrenergic, non-cholinergic nerve stimulation. Neurosci.5, 853–859 (1980).CrossRefGoogle Scholar
  33. [33]
    T. Tomita,Electrical activity (spikes and slow waves) in gastrointestinal muscle. InSmooth Muscle: An Assessment of Current Knowledge (Eds. E. Bulbring, A. F. Brading, A. W. Jones and T. Tomita) Edward Arnold Publishers Ltd, London 1981.Google Scholar
  34. [34]
    I. A. Vladimirova and M. F. Shuba,The effect of strychnine, hydrastin, and apamin on synaptic transmission in smooth muscle cells. Neurofiziologiya10, 295–299 (1978).Google Scholar
  35. [35]
    D. P. Westfall, G. K. Hogaboom, J. Colby, J. P. O'Donnell and J. S. Fedan,Direct evidence against a role of ATP as the nonadrenergic, noncholinergic inhibitory neurotransmitter in guinea pig taenia coli. Proc. Nat. Acad. Sci.79, 7041–7045 (1982).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • M. E. Zacour
    • 1
  • B. Collier
    • 1
  • J. G. Martin
    • 1
  1. 1.Meakins-Christie Laboratories and Dept of Pharmacology and Experimental TherapeuticsMcGill UniversityMontrealCanada

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