Agents and Actions

, Volume 22, Issue 1–2, pp 43–49 | Cite as

Effects of low molecular weight fibrin degradation products 6A and 6D on rabbit aorta strips

  • F. Marceau
  • J. Bouthillier
  • B. Tremblay
  • S. St.-Pierre
Histamine and Kinins


Two small molecular weight fibrin degradation product, the pentapeptide 6A and the undecapeptide 6D, produced relaxations of norepinephrine-contracted rabbit aorta strips. The relaxations were slow-developing and were elicited by both peptides at supramicromolar concentrations; the amplitude of relaxations were small for 6D. The relaxations induced by 6A were not dependent on the presence of endothelium and were not modified by a mixture of indomethacin, pyrilamine, and cimetidine. The amplitude of the relaxations produced by 6A and 6D increased as a function of incubation timein vitro.

In another experimental system, peptides 6A and 6D failed to increase 6-keto-PGF release from cultured human umbilical endothelial cells. Histamine and bradykinin were both active in this system.


Peptide Molecular Weight Endothelial Cell Histamine Indomethacin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    B. Gerdin and T. Saldeen,Effect of fibrin degradation products on microvascular permeability. Thromb. Res.13, 995–1006 (1978).CrossRefPubMedGoogle Scholar
  2. [2]
    M. Belew, B. Gerdin, J. Porath and T. Saldeen,Isolation of vasoactive peptides from human fibrin and fibrinogen degraded by plasmin. Thromb. Res.13, 983–994 (1978).CrossRefPubMedGoogle Scholar
  3. [3]
    B. Gerdin, M. Belew, O. Lindquist and T. Saldeen,Effect of a fibrin-derived peptide on pulmonary microvascular permeability. In:The microembolism syndrome. (Ed. T. Saldeen), pp. 233–239, Almqvist & Wiskell International, Stockholm 1979.Google Scholar
  4. [4]
    M. Belew, B. Gerdin, G. Linddeberg, J. Porath, T. Saldeen and R. Walling,Structure-activity relationships of vasoactive peptides derived from fibrin or fibrinogen degraded by plasmin. Biochim. Biophys. Acta.621, 169–178 (1980).PubMedGoogle Scholar
  5. [5]
    B. Gerdin, L. Juhlin and T. Saldeen,Cutaneous reactions to two fibrin-derived peptides. Acta Dermatovener.61, 558–560 (1981).Google Scholar
  6. [6]
    R. G. G. Andersson, K. Saldeen and T. Saldeen,A fibrin(ogen) derived pentapeptide induces vasodilation, prostacyclin release and an increase in cyclic AMP. Thromb. Res.30, 213–218 (1985).CrossRefGoogle Scholar
  7. [7]
    J. Mehta, T. Wargovich, W. W. Nichols, K. Saldeen, R. Wallin and T. Saldeen,Peptide 6A, a fibrin(ogen) degradation product, increases coronary blood flow. Am. J. Physiol.249, H457-H462 (1985).PubMedGoogle Scholar
  8. [8]
    T. Saldeen, J. W. Ryan and P. Berryer,A peptide derived from fibrin(ogen) inhibits angiotensin converting enzyme and potentiates the effects of bradykinin. Thromb. Res.23, 465–470 (1981).CrossRefPubMedGoogle Scholar
  9. [9]
    D. Regoli and J. Barabé,Pharmacology of bradykinin and related kinins. Pharmacol. Rev.32, 1–46 (1980).PubMedGoogle Scholar
  10. [10]
    T. E. Hugli and F. Marceau,Effects of the C5a anaphylatoxin and its relationship to cyclo-oxygenase metabolites in rabbit vascular strips. Br. J. Pharmacol.84, 725–733 (1985).PubMedGoogle Scholar
  11. [11]
    C. Lundberg, F. Marceau, R. Huey and T. E. Hugli,Anaphylatoxin C5a fails to promote prostacyclin release in cultured endothelial cells from human umbilical veins. Immunopharmacology12, 135–143 (1986).CrossRefPubMedGoogle Scholar
  12. [12]
    R. B. Merrifield,Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. J. Am. Chem. Soc.85, 2149–2154 (1963).CrossRefGoogle Scholar
  13. [13]
    A. R. Mitchell, S. B. H. Kent, M. Engelgard and R. B. Merrifield,A new synthetic route to tertbutyloxycarbonylaminoacyl-4-(oxymethyl) phenylacetamidomethylresin, an improved support for solid phase peptide synthesis. J. Org. Chem.43, 2845–2852 (1978).CrossRefGoogle Scholar
  14. [14]
    B. F. Gisin,Preparation of Merrifield resins through total esterification with caesium salts. Helv. Chim. Acta56, 1476–1482 (1973).CrossRefGoogle Scholar
  15. [15]
    S. A. St-Pierre, J. M. Lalonde, M. Gendreau, R. Quirion, D. Regoli and F. Rioux,Synthesis of peptides by the solid-phase method. 6. Neurotensin, fragments and analogs. J. Med. Chem.24, 370–376 (1981).CrossRefPubMedGoogle Scholar
  16. [16]
    W. Koenig and R. Geiger,New method for the synthesis of peptides: activation of the carbonyl group with dicyclohexylcarbodiimide by using 1-hydroxybenzotriazole as additive. Chem. Ber.103, 788–798 (1970).PubMedGoogle Scholar
  17. [17]
    E. Kaiser, R. L. Colescot, C. D. Bossinger and P. I. Cook,Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal. Biochem.34, 595–598 (1970).CrossRefPubMedGoogle Scholar
  18. [18]
    R. F. Furchgott and S. Bhadrakom,Reactions of strips of rabbit aorta to epinephrine, isopropylarterenol, sodium nitrite and other drugs. J. Pharmacol. Exp. Ther.108, 129–143 (1953).PubMedGoogle Scholar
  19. [19]
    F. Marceau and T. E. Hugli,Effect of C3a and C5a anaphylatoxins on guinea pig blood vessels. J. Pharmacol. exp. Ther.230, 749–754 (1985).Google Scholar
  20. [20]
    R. F. Furchgott,Role of endothelium in responses of vascular smooth muscle. Circ. Res.53, 557–573 (1983).PubMedGoogle Scholar
  21. [21]
    M. Eriksson, K. Saldeen, T. Saldeen, K. Strandberg and R. Wallin,Fibrin-derived vasoactive peptides release histamine. Int. J. Microcirc. Clin. Exp.2, 337–345 (1983).PubMedGoogle Scholar
  22. [22]
    S. L. Hong,Effect of bradykinin and thrombin on prostacyclin synthesis in endothelial cells from calf and pig aorta and human umbilical cord vein. Thromb. Res.18, 787–795 (1980).CrossRefPubMedGoogle Scholar
  23. [23]
    F. Alhenc-Gelas, F. S. J. Tsai, K. S. Callahan, W. B. Campbell and A. R. Johnson,Stimulation of prostaglandin formation by vasoactive mediators in cultured human endothelial cells. Prostaglandins24, 723–742 (1982).CrossRefPubMedGoogle Scholar
  24. [24]
    U. Forstermann, G. Hertting and B. Neufang,The importance of endogenous prostaglandins other than prostacyclin for the modulation of contractility of some rabbit blood vessels. Br. J. Pharmacol.81, 623–630 (1984).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • F. Marceau
    • 1
  • J. Bouthillier
    • 1
  • B. Tremblay
    • 1
  • S. St.-Pierre
    • 2
  1. 1.Unité de Recherche sur l'Inflammation et en Immunologie et RhumatologieC.H.U.L.Canada
  2. 2.INRS-SantéPavillon GamelinMontréalCanada

Personalised recommendations