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A heat-inducibleAdh gene as a reporter gene for a negative selection in transgenicArabidopsis

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Abstract

Chimaeric genes were constructed containing theArabidopsis alcohol dehydrogenase gene under the control of different soyabean heat shock promoters. TransgenicArabidopsis plants, derived from theAdh-null mutant R003 by transformation with the different constructs, showed heat-inducible expression of ADH in leaves. Different levels were generated using the different constructs; transcriptional and translational fusions with theGmhsp 17.6-L heat shock gene were most efficient. Repeated cycles of heat stress and recovery periods increased the ADH levels significantly. Constitutive background levels of ADH activity were negligible in these plants but heat-inducibility was significantly reduced during imbibition and early stages of seed germination. The induction of high levels of ADH activity after 4–6 days following imbibition and vernalization caused susceptibility to a subsequent treatment with allyl alcohol. The use of ectopic expression ofAdh as a selection marker for the isolation of mutants of signal transduction systems is discussed.

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  1. Klaus Severin

    Present address: Institut für Pharmazeutische Biologie, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Germany

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  1. Lehrstuhl für Genetik, Universität Tübingen, Auf der Morgenstelle 28, D-72076, Tübingen, Germany

    Klaus Severin, Armin Wagner & Fritz Schöffl

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  1. Klaus Severin
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  2. Armin Wagner
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  3. Fritz Schöffl
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Severin, K., Wagner, A. & Schöffl, F. A heat-inducibleAdh gene as a reporter gene for a negative selection in transgenicArabidopsis . Transgenic Research 4, 163–172 (1995). https://doi.org/10.1007/BF01968781

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  • DOI: https://doi.org/10.1007/BF01968781

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