Detection of tetracycline-resistant strains ofUreaplasma urealyticum by hybridization assays
Five wild-type strains of tetracycline-resistantUreaplasma urealyticum and one tetracycline-sensitiveUreaplasma urealyticum strain were tested for presence of thetetM sequence by hydridization assays. Two sorts of probes were used, a plasmid containing thetetM fragment and thetetM fragment alone. Two sorts of label were used for each, digoxigenin and radio-labelling. Both purified DNA at various concentrations and non-purified DNA in the pellet from a 10 ml culture were used as targets. The digoxigenin-labelled whole plasmid probe was as specific as the insert alone when hybridized with non-purified DNA, and was more sensitive than radio-labelled probes.
KeywordsInternal Medicine Hybridization Assay Plasmid Probe Urealyticum Strain
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