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Detection of tetracycline-resistant strains ofUreaplasma urealyticum by hybridization assays

  • B. Brunet
  • B. de Barbeyrac
  • H. Renaudin
  • C. Bébéar
Notes

Abstract

Five wild-type strains of tetracycline-resistantUreaplasma urealyticum and one tetracycline-sensitiveUreaplasma urealyticum strain were tested for presence of thetetM sequence by hydridization assays. Two sorts of probes were used, a plasmid containing thetetM fragment and thetetM fragment alone. Two sorts of label were used for each, digoxigenin and radio-labelling. Both purified DNA at various concentrations and non-purified DNA in the pellet from a 10 ml culture were used as targets. The digoxigenin-labelled whole plasmid probe was as specific as the insert alone when hybridized with non-purified DNA, and was more sensitive than radio-labelled probes.

Keywords

Internal Medicine Hybridization Assay Plasmid Probe Urealyticum Strain 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • B. Brunet
    • 1
  • B. de Barbeyrac
    • 1
  • H. Renaudin
    • 1
  • C. Bébéar
    • 1
  1. 1.Laboratoire de BacteriologieHopital PellegrinBordeaux CedexFrance

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