Agents and Actions

, Volume 24, Issue 1–2, pp 189–195 | Cite as

Lack of involvement of leukotriene and platelet activating factor in passive cutaneous anaphylaxis in rats

  • M. Taira
  • S. W. Kohno
  • H. Yamamura
  • K. Ohata
Platelets and Thrombosis


Possible chemical mediators contributing to 48 hour passive cutaneous anaphylaxis (PCA) in rats were investigated. Fortyeight hour PCA was inhibited considerably by mepyramine and methysergide given intravenously, a finding suggestive of a major role for histamine and serotonin in the reaction. AA-861, a selective 5-lipoxygenase inhibitor did not inhibit the PCA, and leukotriene (LT) D4 or LTE4 and the combination with prostaglandin (PG) E2 had no significant skin reaction. In addition, only small amounts of slow reacting substance of anaphylaxis (SRS-A) were detected in skin fragments,in vitro. Although CV-3988, a selective platelet activating factor (PAF) antagonist, dose-dependently inhibited the PAF-induced skin reaction, the PCA was not affected by treatment with this compound. Indomethacin also had no inhibitory activity on PCA. Thus, sulfidopeptide LTs, PAF and arachidonate cyclooxygenase metabolites probably do not contribute to PCA, at least in rats.


Histamine Indomethacin Platelet Activate Factor Skin Reaction Arachidonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



dinitrophenylated Ascaris suum extract




mast cell medium


platelet activating factor


passive cutaneous anaphylaxis




slow reacting substance of anaphylaxis


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Z. E. Mielens, E. W. Ferguson and F. J. Rosenberg,Effect of anti-anaphylactic drugs upon passive cutaneous anaphylaxis mediated by graded doses of reaginic or non-reaginic antibodies in rats, Int. Arch. Allergy47, 633–649 (1966).Google Scholar
  2. [2]
    B. N. Halpern, T. Neveu and S. Spector,On the nature of chemical mediators involved in anaphylactic reactions in mice, Br. J. Pharmacol20, 389–398 (1963).Google Scholar
  3. [3]
    R. A. Lewis and K. F. Austen,Mediation of local homeostasis and inflammation by leukotrienes and other mast cell-dependent compounds, Nature293, 103–108 (1981).PubMedGoogle Scholar
  4. [4]
    M. Chignard, J. P. Couedic, P. Anderson and C. Brange,Use of steroidal antiinflammatory drug provides further evidence for a potential role of PAF-acether in bronchial anaphylaxis, Int. Archs Allergy appl. Immun.81, 184–185 (1986).Google Scholar
  5. [5]
    T. Tada and K. Okumura,Regulation of homocytotropic antibody formation in the rat. I. Feed-back regulation by passively administered antibody, J. Immunol.106, 1002–1011 (1971).PubMedGoogle Scholar
  6. [6]
    H. N. Eisen, S. Belman and M. E. Carsten,The reaction of 2,4-dinitrobenzenesulfonic acid with free amino groups of proteins, J. Amer. Chem. Soc.75, 4583–4585 (1953).Google Scholar
  7. [7]
    R. P. Orange and E. G. Moore,The effect of thiols on the immunologic release of slow reacting substance of anaphylaxis, J. Immunol.116, 392–397 (1976).PubMedGoogle Scholar
  8. [8]
    C. D. May, M. Lyman, R. Alberto and J. Cheng,Procedures for immunochemical study of histamine release from leukocytes with samll volume of blood, J. Allergy46, 12–20 (1970).PubMedGoogle Scholar
  9. [9]
    S. Watanabe-Kohno and C. W. Parker,Role of arachidonic acid in the biosynthesis of slow reacting substance of anaphylaxis (SRS-A) from sensitized guinea pig lung fragments: Evidence that SRS-A is very similar or identical structurally to nonimmunologically induced forms of SRS, J. Immunol.125, 946–955 (1980).PubMedGoogle Scholar
  10. [10]
    K. Rahman, T. Nagatsu and T. Kato,New and highly sensitive assay for 1–5-hydroxytryptophan decarboxylase activity by high-performance liquid chromatography-voltammetry, J. Chromatogr.221, 265–270 (1980).PubMedGoogle Scholar
  11. [11]
    S. Katayama, H. Shionoya and S. Ohtake,A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous anaphylaxis in guinea pigs and rats, Microbiol. Immunol.22, 89–101 (1978).PubMedGoogle Scholar
  12. [12]
    R. P. Orange, J. Stechschulte and K. F. Austen,Cellular mechanisms involved in the release of slow reacting substance of anaphylaxis, Fed. Proc.28, 1710–1715 (1969).PubMedGoogle Scholar
  13. [13]
    P. Crunkhorn and A. L. Willis,Cutaneous reactions to intradermal prostaglandins, Br. J. Pharmacol.41, 49–56 (1971).PubMedGoogle Scholar
  14. [14]
    A. Ueno, K. Tanaka, M. Katori, M. Hayashi and Y. Arai,Species difference in increased vascular permeability by synthetic leukotriene C 4 and D 4, Prostaglandins21, 637–648 (1981).PubMedGoogle Scholar
  15. [15]
    S. Hwang, C. Li, M. Lam and T. Y. Shen,Characterization of cutaneous vascular permeability induced by platelet-activating factor in guinea pigs and rats and its inhibition by a platelet-activating factor receptor antagonist, Lab. Invest.52, 617–630 (1985).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • M. Taira
    • 1
  • S. W. Kohno
    • 1
  • H. Yamamura
    • 1
  • K. Ohata
    • 1
  1. 1.Department of PharmacologyKyoto Pharmaceutical University MisasagiYamashina, KyotoJapan

Personalised recommendations