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, Volume 24, Issue 1–2, pp 189–195 | Cite as

Lack of involvement of leukotriene and platelet activating factor in passive cutaneous anaphylaxis in rats

  • M. Taira
  • S. W. Kohno
  • H. Yamamura
  • K. Ohata
Platelets and Thrombosis

Abstract

Possible chemical mediators contributing to 48 hour passive cutaneous anaphylaxis (PCA) in rats were investigated. Fortyeight hour PCA was inhibited considerably by mepyramine and methysergide given intravenously, a finding suggestive of a major role for histamine and serotonin in the reaction. AA-861, a selective 5-lipoxygenase inhibitor did not inhibit the PCA, and leukotriene (LT) D4 or LTE4 and the combination with prostaglandin (PG) E2 had no significant skin reaction. In addition, only small amounts of slow reacting substance of anaphylaxis (SRS-A) were detected in skin fragments,in vitro. Although CV-3988, a selective platelet activating factor (PAF) antagonist, dose-dependently inhibited the PAF-induced skin reaction, the PCA was not affected by treatment with this compound. Indomethacin also had no inhibitory activity on PCA. Thus, sulfidopeptide LTs, PAF and arachidonate cyclooxygenase metabolites probably do not contribute to PCA, at least in rats.

Keywords

Histamine Indomethacin Platelet Activate Factor Skin Reaction Arachidonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DNP-As

dinitrophenylated Ascaris suum extract

LT

leukotriene

MCM

mast cell medium

PAF

platelet activating factor

PCA

passive cutaneous anaphylaxis

PG

prostaglandin

SRS-A

slow reacting substance of anaphylaxis

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Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • M. Taira
    • 1
  • S. W. Kohno
    • 1
  • H. Yamamura
    • 1
  • K. Ohata
    • 1
  1. 1.Department of PharmacologyKyoto Pharmaceutical University MisasagiYamashina, KyotoJapan

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