Advertisement

Agents and Actions

, Volume 24, Issue 1–2, pp 87–94 | Cite as

Histamine induced murine suppressor and potentiating factors

  • R. Eckert
  • H. Repke
  • E. Rentz
  • M. Nausch
  • H. Friemel
Histamine and Kinins

Abstract

Mouse spleen cells were incubated for 24 hours in the presence of histamine (10−13–10−3M). Aliquots of the histamine free supernatants were intravenously injected into either syngenic or allogenic mice which were subsequently analysed by the Jerne plaque assay in respect of their specific IgM response against red blood cells from sheep.

Depending on the histamine concentration during the preincubation and the mouse strain, the effects of the supernatants were found to be suppressive or potentiating. Both the histamine induced suppressor and potentiating factor are generated by T-cells and can be precipitated by (NH4)2SO4. These precipitates were fractionated by gel filtration which revealed a molecular weight of both factors in the range of 20 to 50 kDa. Both lymphokines were found to be sensitive to trypsin, low pH, and heat treatment but were not affected by neuraminidase. They do only interfere with the early events of the IgM response within 24 hours after the administration of the antigen. The conclusion is that the bidirectional modulation of the humoral immune response by histamine in mice is partially due to the induction of these lymphokines by a different efficacy of the histamine receptor activation at different T-cell subsets.

Keywords

Histamine Trypsin Heat Treatment Spleen Cell Humoral Immune Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    D. J. Beer, S. M. Matloff and R. E. Rocklin,The influence of histamine on immune and inflammatory response, Adv. Immunol.35, 209–268 (1984).PubMedGoogle Scholar
  2. [2]
    S. Schnitzler, R. Eckert, D. Volk and R. Grunow,Histamine and immune reactions. I. Histamine receptor bearing lymphocytes. Allerg. Immunol.28, 219–235 (1982).Google Scholar
  3. [3]
    S. Suzuki and R. Huchet,Properties of a histamine-induced suppressor factor in the regulation of lymphocyte response to PHA in mice, Cell. Immunol.68, 349–358 (1982).PubMedGoogle Scholar
  4. [4]
    R. Eckert and H. Repke,Histamine as endogenous immunomodulator, Meth. and Find. Exptl. Clin. Pharmacol.8, 183–187 (1986).Google Scholar
  5. [5]
    R. E. Rocklin, A. Blidy and M. Kamal,Physicochemical characterization of human histamine-induced suppressor factor, Cell. Immun.76, 243–252 (1983).Google Scholar
  6. [6]
    J. N. Siegel, A. Schwartz, P. W. Askenase and R. K. Gershon,T-cell suppression and contrasuppression induced by histamine H 2-and H 1-receptor agonists, respectively, Proc. Natl. Acad. Sci. USA79, 5052–5056 (1982).PubMedGoogle Scholar
  7. [7]
    R. Eckert, E. Reichelt, S. Gruner, A. Stöss and S. Schnitzler,Histamine receptor bearing lymphocytes. IV. Differential effect of histamine on cell-mediated and antibody-mediated immune response, Biomed. Biochim. Acta42, 301–303 (1983).PubMedGoogle Scholar
  8. [8]
    R. Eckert, H. Repke and E. Reichelt,Modulation of the adoptive immune response in mice by histamine, Agents and Actions24, 80–86 (1988).PubMedGoogle Scholar
  9. [9]
    R. Eckert, E. Mix and B. v. Broen,Studies on column fractionation of immune cells. VI. Separation of thymus derived lymphocytes by means of protein beads coated with antigen-antibody-complexes, Acta biol. med. germ.35, 663–671 (1976).PubMedGoogle Scholar
  10. [10]
    E. Rentz and W. Diezel,Influences of thymic hormones on mitogen-induced interferon production in lymphocytes. I. Augmentation of mitogen-induced immune interferon production by thymosins, Arch. Geschwulstforsch.53, 547–550 (1983).PubMedGoogle Scholar
  11. [11]
    H. Friemel and M. Nausch,Interleukin 1 determination, Allerg. Immunol.31, 245–257 (1985).Google Scholar
  12. [12]
    M. Iwata, M. Akasaki and K. Ishizaka,Modulation of the biological activation of IgE binding factors. VI. The activation of phospholipase by glycosylation enhancing factor, J. Immunol.133, 1505–1512 (1984).PubMedGoogle Scholar
  13. [13]
    M. M. Khan, P. Sansoni, E. G. Engleman and K. L. Melmon,Pharmacologic effects of autacoids on subsets of T-cells. Regulation of expression/function of histamine-2 receptors by a subset of suppressor cells, J. Clin. Invest.75, 1578–1583 (1985).PubMedGoogle Scholar
  14. [14]
    T. P. Kenakin,The classification of drugs and drug receptors in isolated tissues, Pharmacol. Reviews36, 165–222 (1984).Google Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • R. Eckert
    • 1
  • H. Repke
    • 2
  • E. Rentz
    • 3
  • M. Nausch
    • 4
  • H. Friemel
    • 4
  1. 1.Institute of Clinical ImmunologyHumboldt UniversityBerlinGerman Democratic Republic
  2. 2.Institute of Drug ResearchAcademy of Sciences of the GDRBerlinGerman Democratic Republic
  3. 3.Central Institute of Cancer ResearchAcademy of Sciences of the GDRBerlinGerman Democratic Republic
  4. 4.Institute of ImmunologyWilhelm Pieck UniversityRostockGerman Democratic Republic

Personalised recommendations