Acute inflammatory responses in rats with protein-calorie malnutrition
An animal model of protein-calorie malnutrition (PCM) was produced by keeping rats on a protein-free diet during the 13-day interval which followed the period of normal lactation. Malnutrition was characterized by reduction in body weight, in plasma proteins, cholesterol, haemoglobin and leucocyte counts, relative to controls from the same litter receiving a balanced diet.
Acute inflammatory responses induced in these animals by carrageenin or staphylococci suspensions, differed markedly from those evoked in matching controls: the resulting oedema developed more slowly and yet persisted for longer intervals; Evans blue, injected i.v., leaked into the inflamed areas in smaller quantities; reduced cell migration to the site of the lesion was observed.
The slow development of the response was attributed to the decreased levels of circulating proteins and its persistency to a defective drainage of exuded material.
It is concluded that PCM can impair the capacity of response to noxious stimuli, thus rendering the animals more susceptible to infections.
KeywordsCholesterol Body Weight Animal Model Cell Migration Plasma Protein
Unable to display preview. Download preview PDF.
- P. Kulapongs, R. Edelman, R. Suskind andR.E. Olson,Defective Local Leukocyte Mobilization in Children with Kwashiorkor, Am. J. Nutr.30, 367 (1977).Google Scholar
- E.F. Collares andJ.R. Woiski,Estudo de atividade específica da lactase, invertase e maltase em mucosa de intestino delgado de ratos submetidos à má nutrição Protêica, Arq. Gastroent. (São Paulo)9, 191 (1972).Google Scholar
- W. Horwitz,Official Methods of Analysis of the Association of Official Agricultural Chemistry, 10th ed. (Washington, D.C. 1975).Google Scholar
- L. Manna andS.M. Hauge,A Possible Rationship of Vitamin B 13 to Orotic Acid, J. biol. Chem.202, 91 (1952).Google Scholar
- C.V. Winder, J. Wax andM.A. Been,Rapid Foot Volume Measurements on Unanaesthetized Rats, and the Question of a Phenylbutazone Effect on Anaphylactoid Edema, Arch. int. Pharmacodyn.112, 174 (1975).Google Scholar
- R.A. Rawson,The Binding of T-1824 and Structurally Related Diazo-Dyes by the Plasma Protein, Am. J. Physiol.138, 708 (1942).Google Scholar
- M. Rocha e Silva andJ. Garcia-LLeme,Chemical Mediators of the Acute Inflammatory Reaction (Pergamon Press, Oxford 1972).Google Scholar
- A.G. Gornall, C.J. Bardawill andM.M. David,Determination of Serum Proteins by Means of the Biuret Reaction, J. biol. Chem.177, 751 (1949).Google Scholar
- R. Richterich,Clinische Chemie, Theorie und Praxis (S. Karger, Basel 1965).Google Scholar
- E.M. Darmady andS.G.T. Davenport,Haematological Technique, 3rd ed. (J. & A. Churchill, London 1963).Google Scholar
- M.R. Leme-Brasil, E.F. Collares, F.C. Soares andJ.R. Woiski,Atividade total de dissacaridases em intestino dèlgado de ratos submetidos à carência protêica experimental, Arq. Gastroent.12, 165 (1965).Google Scholar
- F. Gomez, R.R. Galvin, J. Cravioto andS. Frenk,Malnutrition in Infancy and Childhood, with special Reference to Kwashiorkor Adv.Pediatrics 7, 131 (1955).Google Scholar
- J. Garcia-Leme, J. Hamamura, M.P. Leite andM. Rocha e Silva,Pharmacological Analysis of the Acute Inflammatory Process Induced in the Rat's Paw by Local Injection of Carrageenin and by Heating, Brit. J. Pharmacol.48, 88 (1973).Google Scholar
- G.P. Lewis,Changes in the Composition of Rabbit Hind Limb Lymph after Thermal Injury, J. Physiol. 2206,619 (1960).Google Scholar
- S. Boyles, G.P. Lewis andB. Westcott,Changes in the Composition of Hind Limb Lymph after chemical Injury, Brit. J. Pharmac.39, 798 (1970).Google Scholar
- K. Morsdorf,The Inhibition of Protein Catabolism by Anti-inflammatory Drugs, Biochemistry and Drug interaction (eds. A. Bertelli and J.C. Houck; Excepta Medica, Amsterdam, 1969).Google Scholar
- N.B. Piller,A Comparison of the Effect of Benzopyrenes and Other Drugs with anti-Inflammatory Properties on Acid and Neutral Protease Activity Levels in Various Tissues After Thermal Injury, Br. J. exp. Path.57, 411 (1976).Google Scholar
- F. Gomez, R.R. Galvan, J. Cravioto andS. Frenk,Studies of the Undernourished Child. XI. Enzymatic Activity of the Duodenal Contents in Children Affected with Third Degree Malnutrition, Pediatrics,13, 548 (1954).Google Scholar