Binding of [3H]cimetidine to rat brain tissue
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Binding of [3H]cimetidine to rat brain tissue was investigated, and a saturable binding with dissociation constant 0.22±0.05 μM found. This binding is inhibited by a range of imidazole-derived histamine H2-receptor antagonists, but not by a number of non-imidazole H2-receptor antagonists. It is concluded that the [3H]cimetidine binding site in rat brain tissue that is labelled in these experiments is not the histamine H2-receptor.
KeywordsBinding Site Histamine Brain Tissue Cimetidine Dissociation Constant
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