Prevalence of antibodies toBorrelia burgdorferi in forestry workers and blood donors from the same region in Switzerland

  • D. Nadal
  • W. Wunderli
  • H. Briner
  • K. Hansen


Sera from 259 forestry workers and 100 blood donors in the Canton of Solothurn, Switzerland, were tested for IgG antibodies toBorrelia burgdorferi in two EIAs using as antigen either sonic extract of whole organisms or purified flagella. Applying a 95% specific cut-off value based on results in the sera of 100 blood donors, 86 (33%) and 91 (35%) of the forestry workers respectively showed an elevated specific IgG level in the two EIAs. None of the 259 forestry workers had clinical signs of active infection at the time blood was taken, and only nine could recall experiencing erythema-migrans-like skin lesions within the last ten years. Thus, asymptomatic infections must be frequent. Elevated specific antibody levels increased significantly with the age of the forestry workers (p<0.0001) and the duration of occupational exposure to ticks (p=0.0001). Thus serological results in individuals with high exposure to ticks must be interpreted with caution in view of the high a priori prevalence of antibodies toBorrelia burgdorferi in such persons. The prevalence of antibody to theBorrelia burgdorferi flagellum in a control population not selected for tick exposure, in this case blood donors, seems to be independent of geographical origin.


Skin Lesion Blood Donor Occupational Exposure Geographical Origin Antibody Level 
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  1. 1.
    Burgdorfer, W., Barbour, A. G., Hayes, S. F., Benach, J. L., Grunwaldt, E., Davis, J. P. Lyme disease — a tick-borne spirochetosis? Science 1982, 216: 1317–1319.PubMedGoogle Scholar
  2. 2.
    Craft, J. K., Grodzicki, R. L., Steere, A. C. Antibody response in Lyme disease: evaluation of diagnostic tests. Journal of Infectious Diseases 1984, 149: 789–795.PubMedGoogle Scholar
  3. 3.
    Hansen, K., Hindersson, P., Pedersen, N. S. Measurement of antibodies to theBorrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. Journal of Clinical Microbiology 1988, 26: 338–346.PubMedGoogle Scholar
  4. 4.
    Münchhoff, P., Wilske, B., Preac-Mursic, V., Schierz, G. Antibodies againstBorrelia burgdorferi in Bavarian forest workers. Zentralblatt für Bakteriologie und Hygiene (A) 1986, 263: 412–419.Google Scholar
  5. 5.
    Guy, E. C., Bateman, D. E., Martyn, C. N., Heckels, J. E., Lawton, N. F. Lyme disease: prevalence and clinical importance ofBorrelia burgdorferi specific IgG in forestry workers. Lancet 1989, i: 484–486.CrossRefGoogle Scholar
  6. 6.
    Fahrer, H., Sauvain, M. J., van der Linden, S., Zihoua, E., Gern, L., Aeschlimann, A. Prävalenz der Lyme-Borreliose in einer schweizerischen Risikopopulation. Schweizerische medizinische Wochenschrift 1988, 118: 65–69.PubMedGoogle Scholar
  7. 7.
    Schmutzhard, E., Stanek, G., Pletschette, M., Hirschl, A. M., Pallua, A., Schmitzberger, R., Schlögl, R. Infections following tickbites. Tick-borne encephalitis and Lyme borreliosis — a prospective epidemiological study from Tyrol. Infection 1988, 16: 269–272.CrossRefPubMedGoogle Scholar
  8. 8.
    Paul, H., Gerth, H.-J., Ackermann, R. Infectiousness for humans ofIxodes ricinus containingBorrelia burgdorferi. Zentralblatt für Bakteriologie und Hygiene (A) 1986, 263: 412–419.Google Scholar
  9. 9.
    Hansen, K., Asbrink, E. Serodiagnosis of erythema migrans and acrodermatitis chronica atrophicans by theBorrelia burgdorferi flagellum enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 1989, 27: 545–551.PubMedGoogle Scholar
  10. 10.
    Schmutzhard, E., Pohl, P., Stanek, G. Borrelia burgdorferi antibodies in patients with relapsing/remitting form and chronic progressive form of multiple sclerosis. Journal of Neurology, Neurosurgery, and Psychiatry 1988, 51: 1215–1218.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft MbH 1989

Authors and Affiliations

  • D. Nadal
    • 1
  • W. Wunderli
    • 2
  • H. Briner
    • 1
  • K. Hansen
    • 3
  1. 1.Division of Infectious Diseases, Department of PediatricsUniversity of ZurichZurichSwitzerland
  2. 2.Department of Virology and ImmunologyUniversity of ZurichZurichSwitzerland
  3. 3.Borrelia Laboratory, Statens SeruminstitutCopenhagenDenmark

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