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Analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures

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Abstract

The autoagglutination phenomenon (AA) was studied in 71 strains of mesophilic aeromonads isolated from stool cultures of patients with acute diarrhea (28Aeromonas hydrophila, 20Aeromonas sobria, and 23Aeromonas caviae). Two phenotypes were considered in the AA+ strains: SP+ (self-pelleting) and PAB+ (precipitation after boiling). Serogrouping of the strains was performed only with the 0:11 serum. Other properties studied were agglutination with acriflavine (Acr) and crystal violet uptake (CVB). Phenotype AA+ was found in 29.5 % of the strains; 7 % of these AA+ strains ecxhibited the SP+ PAB+ pattern and 22.5 % the SP− PAB+ pattern. The speciesAeromonas hydrophila comprised the highest proportion (28.5 %), andAeromonas caviae the lowest (13 %). Fifty percent of the PAB+ strains ofAeromonas hydrophila andAeromonas sobria belonged to serotype 0:11. None of the PAB+ strains ofAeromonas caviae belonged to this serotype. 8.4 % of the strains were Acr+ and 23.9 % CVB+.

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Authors and Affiliations

  1. Servicio de Microbiologia, Hospital Son Dureta, 07014, Palma de Mallorca, Spain

    J. Reina, I. Llompart, J. Gomez, N. Borrell, A. Serra & P. Alomar

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  1. J. Reina
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  2. I. Llompart
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  3. J. Gomez
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  4. N. Borrell
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  5. A. Serra
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  6. P. Alomar
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Reina, J., Llompart, I., Gomez, J. et al. Analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures. Eur. J. Clin. Microbiol. Infect. Dis. 9, 897–901 (1990). https://doi.org/10.1007/BF01967507

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