Skip to main content
SpringerLink
Log in

Analysis of the relationship between bacterial adherence and extracellular production of mannose, galactose, glucose and ribose inStaphylococcus epidermidis andStaphylococcus hominis

  • Article
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

Gas-liquid chromatography-mass spectrometry was used to analyze the extracellular extracts of 108 coagulase-negative staphylococcal strains for the presence of mannose, galactose, glucose and ribose, in order to determine whether production of these four monosaccharides, regarded as potential staphylococcal slime components, was associated with the adherence capacity of the individual strains. A total of 90Staphylococcus epidermidis and 18Staphylococcus hominis strains were studied. Using the quantitative spectrophotometric assay, 21Staphylococcus epidermidis strains were classified as strongly adherent, 12 as moderately adherent, 11 as weakly adherent, and 46 as nonadherent. All 18Staphylococcus hominis strains were nonadherent. Mannose, galactose, glucose and ribose were detected as the main monosaccharide components in the extracellular extracts of all strains examined. Moreover, the mean relative concentrations of these monosaccharides were essentially the same for the different adherence phenotypes within the speciesStaphylococcus epidermidis. These results showed that there was no causal connection between the adherence of coagulase-negative staphylococci and the extracellular production of any of the four monosaccharides analyzed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lowy FD, Hammer SM:Staphylococcus epidermidis infections. Annals of Internal Medicine 1983, 99: 834–839.

    PubMed  Google Scholar 

  2. Ponce De Leon S, Wenzel RP: Hospital-acquired bloodstream infections withStaphylococcus epidermidis. American Journal of Medicine 1984, 77: 639–644.

    Article  PubMed  Google Scholar 

  3. Bayston R: A model of catheter colonisation in vitro and its relationship to clinical catheter infections. Journal of Infection 1984, 9: 271–276.

    PubMed  Google Scholar 

  4. Elliott TSJ: Intravascular-device infections. Journal of Medical Microbiology 1988, 27: 161–167.

    PubMed  Google Scholar 

  5. Wade JC, Schimpff SC, Newman KA, Wiernik PH:Staphylococcus epidermidis: an increasing cause of infection in patients with granulocytopenia. Annals of Internal Medicine 1982, 97: 503–508.

    PubMed  Google Scholar 

  6. Bayston R, Penny SR: Excessive production of mucoid substance inStaphylococcus SIIA: a possible factor in colonisation of Holter shunts. Developmental Medicine and Child Neurology 1972, 14, Supplement 27: 25–28.

    Google Scholar 

  7. Christensen GD, Simpson WA, Bisno AL, Beachey EH: Adherence of slime-producing strains ofStaphylococcus epidermidis to smooth surfaces. Infection and Immunity 1982, 37: 318–326.

    PubMed  Google Scholar 

  8. Peters G, Locci R, Pulverer G: Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. Journal of Infectious Diseases 1982, 146: 479–482.

    PubMed  Google Scholar 

  9. Christensen GD, Parisi JT, Bisno AL, Simpson WA, Beachey EH: Characterization of clinically significant strains of coagulase-negative staphylococci. Journal of Clinical Microbiology 1983, 18: 258–269.

    PubMed  Google Scholar 

  10. Christensen GD, Baddour LM, Simpson WA: Phenotypic variation ofStaphylococcus epidermidis slime production in vitro and in vivo. Infection and Immunity 1987, 55: 2870–2877.

    PubMed  Google Scholar 

  11. Peters G: New considerations in the pathogenesis of coagulase-negative staphylococcal foreign body infections. Journal of Antimicrobial Chemotherapy 1988, 21, Supplement C: 139–148.

    PubMed  Google Scholar 

  12. Pfaller MA, Herwaldt LA: Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clinical Microbiology Reviews 1988, 1: 281–299.

    PubMed  Google Scholar 

  13. Ludwicka A, Uhlenbruck G, Peters G, Seng PN, Gray ED, Jeljaszewicz J, Pulverer G: Investigation on extracellular slime substance produced byStaphylococcus epidermidis. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1984, 258: 256–267.

    Google Scholar 

  14. Peters G, Schumacher-Perdreau F, Jansen B, Bey M, Pulverer G: Biology ofStaphylococcus epidermidis extracellular slime. In: Pulverer G, Quie PG, Peters G (ed): Pathogenicity and clinical significance of coagulase-negative staphylococci. G. Fischer Verlag, Stuttgart, 1987, p. 15–31.

    Google Scholar 

  15. Schumacher-Perdreau F, Rotering H, Pulverer G: Cell wall analysis and taxonomy of staphylococci. Zentralblatt für Bakteriologie, Microbiologie und Hygiene (A) 1983, 256: 25–36.

    Google Scholar 

  16. Schumacher-Perdreau F, Jansen B, Peters G, Pulverer G: Typing of coagulase-negative staphylococci isolated from foreign body infections. European Journal of Clinical Microbiology & Infectious Diseases 1988, 7: 270–273.

    Google Scholar 

  17. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH: Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. Journal of Clinical Microbiology 1985, 22: 996–1006.

    PubMed  Google Scholar 

  18. Chambers RE, Clamp JR: An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials. Biochemical Journal 1971, 125: 1009–1018.

    PubMed  Google Scholar 

  19. Bryn K, Jantzen E: Analysis of lipopolysaccharides by methanolysis, trifluoroacetylation, and gas chromatography on a fused-silica capillary column. Journal of Chromatography 1982, 240: 405–413.

    Article  Google Scholar 

  20. Tojo M, Yamashita N, Goldmann DA, Pier GB: Isolation and characterization of a capsular polysaccharide adhesin fromStaphylococcus epidermidis. Journal of Infectious Diseases 1988, 157: 713–722.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, University of Turku, Kiinamyllynkatu 13, 20520, Turku, Finland

    P. Kotilainen, M. K. Viljanen & P. Huovinen

  2. Department of Chemistry and Biochemistry, University of Turku, Kiinamyllynkatu 13, 20520, Turku, Finland

    P. Oksman

  3. Department of Medicine, Turku University Central Hospital, 20520, Turku, Finland

    P. Kotilainen & J. Nikoskelainen

Authors
  1. P. Kotilainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Oksman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. K. Viljanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Nikoskelainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Huovinen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kotilainen, P., Oksman, P., Viljanen, M.K. et al. Analysis of the relationship between bacterial adherence and extracellular production of mannose, galactose, glucose and ribose inStaphylococcus epidermidis andStaphylococcus hominis . Eur. J. Clin. Microbiol. Infect. Dis. 9, 873–879 (1990). https://doi.org/10.1007/BF01967502

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01967502

Keywords

Search

Navigation