Abstract
In 210 strains ofEnterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20µg) + clavulanate (10µg) and cefotaxime (30µg) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrumβ-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sanders, C. C., Sanders, W. E.: Emergence of resistance during therapy with the newerβ-lactam antibiotics: role of inducibleβ-lactamase and implication for the future. Reviews of Infectious Diseases 1983, 5: 639–648
Shah, P. M., Stille, W.:Escherichia coli andKlebsiella pneumoniae strains more susceptible to cefoxitin than to third generation cephalosporins. Journal of Antimicrobial Chemotherapy 1983, 11: 597–598.
Kleibe, C., Nies, B. A., Meyer, J. F., Tolxdorff-Neutzling, R. M., Wiedemann, B.: Evolution of plasmid-coded resistance to broad-spectrum cephalosporins. Antimicrobial Agents and Chemotherapy, 1985, 28: 302–307.
Bauernfeind, A., Hörl, G.: Novel R-factor borne beta-lactamase ofEscherichia coli conferring resistance to cephalosporins. Infection 1987, 15: 257–259.
Sirot, D., Sirot, J., Labia, R., Morand, A., Courvalin, P., Darfeuille-Michaud, A., Perroux, R., Cluzel, R.: Transferable resistance to third-generation cephalosporins in clinical isolates ofKlebsiella pneumoniae. Identification of CTX-1, a novel beta-lactamase. Journal of Antimicrobial Chemotherapy 1987, 20: 323–334.
Brun-Buisson, C., Legrand, P., Philippon, A., Montravers, F., Ansquer, M., Duval, J.: Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistantKlebsiella pneumoniae. Lancet 1987, ii: 302–306.
Kitzis, M. D., Billot-Klein, D., Goldstein, F. W., Williamson, R., Collatz, E., Tan Van Nhieu, G., Carlet, J., Acar, J. F., Gutmann, L.: Dissemination of the novel plasmidmediatedβ-lactamase CTX-1, which confers resistance to broad-spectrum cephalosporins, and its inhibition byβ-lactamase inhibitors. Antimicrobial Agents and Chemotherapy 1988, 32: 9–14.
Jarlier, V., Nicolas, M. H., Fournier, G., Philippon, A.: Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactams inEnterobacteriaceae: hospital prevalence and susceptibility patterns. Reviews of Infectious Diseases 1988, 10: 867–878.
Ben Redjeb, S., Ben Yeghlane, H., Boujnah, A., Philippon, A., Labia, R.: Synergy between clavulanic acid and newer beta-lactams on 9 clinical isolates ofKlebsiella pneumoniae, Escherichia coli andSalmonella typhimurium resistant to third generation cephalosporins. Journal of Antimicrobial Chemotherapy 1988, 21: 263–266.
Petit, A., Sirot, D. L., Chanal, C. M., Sirot, J. L., Labia, R., Gerbaud, G., Cluzel, R. A.: Novel plasmid-mediatedβ-lactamase in clinical isolates ofKlebsiella pneumoniae more resistant to ceftazidime than to other broad-spectrum cephalosporins. Antimicrobial Agents and Chemotherapy 1988, 32: 626–630.
Gutmann, L., Kitzis, M., Tan Van Nhieu, G., Lu, T., Carlet, J., Collatz, E., Williamson, R.: Plasmid-mediated beta-lactamase (TEM-7) involved in resistance to ceftazidime and aztreonam. Reviews of Infectious Diseases 1988, 10: 860–866.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Legrand, P., Fournier, G., Buré, A. et al. Detection of extended broad-spectrum beta-lactamases inEnterobacteriaceae in four French hospitals. Eur. J. Clin. Microbiol. Infect. Dis. 8, 527–529 (1989). https://doi.org/10.1007/BF01967473
Issue Date:
DOI: https://doi.org/10.1007/BF01967473