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FCE 20696, a new synthetic immunomodulator: Immunopharmacological profile

  • Inflammation and Immunomodulation
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Abstract

A preliminary characterization of the immunopharmacological profile of the new immunomodulating agent FCE 20696 (6H,6 [2-(dimethylamino)-ethoxycarbonyl]-dibenzo[b,d]pyran-HCl) was performed in mice.

Single i.p. doses of this chemical, concomitantly given with the antigen, increased the antibody response and decreased the delayed hypersensitivity reaction to a suboptimal dose of SRBC, the active doses ranging from 6.25 to 50 mg/kg. No activity was observed using a full antigen dose. Macrophage cytotoxic activity was enhanced 2–4 days after a single i.p. treatment with 50 and 10 mg/kg. Spleen cell proliferation to T and B mitogens was inhibited by a single dose of 30 mg/kg given i.p. 6 days before the test, or by 10 mg/kg×3 days ending one day before the test. Finally, generation of suppressor cells was enhanced by the compound, given p.o. biweekly for at least 7 weeks, at doses ranging from 0.1 to 10 mg/kg. Collectively taken, these data suggest that FCE 20696 has a broad range of immunomodulating activities and that macrophages and suppressor cells are presumed to be the main targets of its pharmacological activity.

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Abbreviations

SRBC:

sheep red blood cells

DH:

delayed hypersensitivity

MTT:

(3-(4,5-dimethylthiazol-1-yl)-2,5-diphenyl tetrazolium bromide)

Con A:

concanavalin A

PHA:

phytohemagglutinin

LPS:

lipopolysaccharide

CCA:

N-(2-carboxyphenyl)-4-chloroanthranilic acid disodium salt

[3H]TdR:

methyl-3H-Thymidine

CNS:

central nervous system

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Authors and Affiliations

  1. Immunology Line, Farmitalia Carlo Erba, R & D, Erbamont Group, Via Giovanni XXIII, 23, 20014 Nerviano, Milan, Italy

    A. M. Isetta, M. C. Fornasiero, M. Ferrari & D. Trizio

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  1. A. M. Isetta
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  2. M. C. Fornasiero
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  3. M. Ferrari
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  4. D. Trizio
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Isetta, A.M., Fornasiero, M.C., Ferrari, M. et al. FCE 20696, a new synthetic immunomodulator: Immunopharmacological profile. Agents and Actions 28, 283–289 (1989). https://doi.org/10.1007/BF01967416

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  • DOI: https://doi.org/10.1007/BF01967416

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