Abstract
Interleukin-1 (IL-1) is a putative mediator of inflammation released by activated macrophagesin vitro. The IL-1 release by rat macrophages collected either from exudates in pertussis-induced air pouches or from the peritoneum during adjuvant arthritis has been investigated. In air pouch inflammation LPS-stimulated macrophages collected from sensitized animals release more IL-1 than cells from control rats at day 6 after challenge. This enhanced IL-1 release parallels the extent of mononuclear cell migration in the inflammatory lesion. In adjuvant arthritis LPS-stimulated macrophages collected from sensitized animals release more IL-1 than cells from control rats at days 16 and 23 after adjuvant injection. The secondary inflammation in arthritic rats was statistically significant at days 16 to 28. These results indicate that during immunological inflammation macrophages either from the inflamed area or from a non-inflamed region release more IL-1 than control cells. This release parallels the extent of the inflammation and may be important in its pathogenesis.
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References
C. A. Dinarello,Biology of interleukin-1. FASEB J.2, 108–115 (1988).
D. S. Snyder and E. R. Unanue,Corticosteroids inhibit murine macrophage Ia expression and interleukin-1 production. J. Immunol.129, 1803–1805 (1982).
S. L. Kunkel, S. W. Chensue and S. H. Phan,Prostaglandins as endogenous mediators of interleukin-1 production. J. Immunol.136, 186–192 (1986).
C. A. Dinarello, T. Ikejima, S. J. C. Warner, S. F. Orencole, G. Lonnemann, J. G. Cannon and P. Libby,Interleukin-1 induces interleukin-1. I. Induction of circulating interleukin-1 in rabbits in vivo and in human mononuclear cells in vitro. J. Immunol.139, 1902–1910 (1987).
W. Lew, J. J. Oppenheim and K. Matsushima,Analysis of the suppression of IL-1α and IL-1β production in human peripheral blood mononuclear adherent cells by a glucocorticoid hormone. J. Immunol.140, 1895–1902 (1988).
K. Phadke, D. G. Carlson, B. D. Gitter and L. D. Butler,Role of interleukin-1 and interleukin-2 in rat and mouse arthritis models. J. Immunol.136, 4085–4091 (1986).
W. J. Johnson, M. J. Di Martino and N. Hanna,Macrophage activation in rat models of inflammation and arthritis: determination of markers of stages of activation. Cell. Immunol.103, 54–64 (1986).
A. D. Sedgwick, A. R. Moore, A. Y. Al-Duaij, J. C. W. Edwards and D. A. Willoughby,The immune response to pertussis in the 6-day air pouch: a model of chronic synovitis. Br. J. Exp. Path.66, 455–464 (1985).
A. Massone, C. Baldari, S. Censini, M. Bartalini, D. Nucci, D. Boraschi and J. L. Telford,Mapping of biologically relevant sites on human IL-1β using monoclonal antibodies. J. Immunol.140, 3812–3816 (1988).
U. K. Laemmli,Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685 (1970).
A. D. Sedgwick, R. E. Hooke, P. Lees and S. A. May,Modulation of lymphocyte activating factor activity (interleukin-1-like activity) and acute phase proteins in pertussis-induced air pouch inflammation by muramyl-dipeptide. Agents and Actions21, 113–120 (1987).
H. Z. Movat,The inflammatory reaction, Elsevier, Amsterdam, New York, Oxford 1985.
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Becherucci, C., Donati, D., Perretti, M. et al. The release of interleukin-1-like activity by macrophages in two models of immunological inflammation in the rat. Agents and Actions 28, 243–247 (1989). https://doi.org/10.1007/BF01967410
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DOI: https://doi.org/10.1007/BF01967410