Abstract
Histamine is widely distributed in the intestine where it is involved in many pathological reactions. The relations between histamine content, diamine oxidase and histidine decarboxylase activity have been investigated along rat's intestine. Results showed significant variations along the intestine. A correlation was observed between histamine and histidine decarboxylase (p<0.01), both of them being even in the small intestine (11.8±2.3 ng/mg ww and 112.5±21.2 fmoles/hr/mg ww respectively), significantly higher in the caecum (16.3±1.9 and 178±20.1) and significantly smaller in the colon (7.3±1.1 and 65.3±11.5) than in other intestinal segments. Diamine oxydase activity was higher in ileum (30.7±7.2 pmoles/min/mg ww) than in jejunum (17.1±2.8), caecum (4.3±0.8) and colon (2.6±2.7), and could not be linked to histamine content. The results fitted in the hypothesis that histamine in rat intestine is mainly located in mast cells where HDC is probably the main enzyme involved in its modulation.
Similar content being viewed by others
References
W. Lorenz, E. Matejka, A. Schmal, W. Seidel, H. J. Reimann, R. Uhlig and G. Mann,A phylogenetic study on the occurence and distribution of histamine in the gastrointestinal tract and other tissues of man and various animals. Com. Gen. Pharmacol.4, 229–250 (1973).
B. I. Hirschowitz,An update on histamine receptors in the gastrointestinal tract. Dig. Dis. Sci.30, 998–1004 (1985).
R. I. McCabe and P. L. Smith,Effects of histamine and histamine receptor antagonists on ion transport in rabbit descending colon. Am. J. Physiol.247, G411-G418 (1984).
W. Lorenz, R. Meyer, A. Doenicke, A. Schmal, H. J. Reimann, M. Hutzel and E. Werle,On the species specificity of the histamine release from mast cell stores by chremophor-El. Arch. exp. Path. Pharmacol.269, 417–418 (1971).
K. S. Kim, B. Backus, M. Harris and P. Rourke,Distribution of diamine oxydase and imidazole-N-methyltransferase along the gastrointestinal tract. Comp. Biochem. Physiol.31, 137–145 (1969).
J. Kusche, R. Mennigen, L. Leisten and B. Amoei,Elevation of the large bowel histamine concentration by aminoquanidine induced diamine oxydase inhibition. Agents and Actions20, 274–276 (1987).
T. Ishibashi, O. Donis, D. Fitzpatrick, N. S. Lee and H. Fisher,Histamine synthesis and degradation in the chick (Gallus gallus) and in the rat (Rattus rattus). Comp. Biochem. Physiol.64c, 227–228 (1979).
S. C. Chen and H. Fisher,Gastric histamine metabolism and acid secretion in rats as influenced by diet and nutrient content. J. Nutr.113, 379–387 (1983).
B. Lebel,A high-sampling-rate automated continuous-flow fluorometric technique for the analysis of nanogram levels of histamine in biological samples. Anal. Biochem.133, 16–29 (1983).
L. R. Hegstrand,A direct, sensitive microassay for mammalian histidine decarboxylase. Biochem. Pharmacol.34, 3711–3716 (1985).
J. Kusche, H. Richter, R. Hesterberg, J. Schmidt and W. Lorenz,Comparison of the 14 C-Putrescine Assay with the NADH Test for the determination of diamine oxydase: description of a standard procedure with a high precision and an improved accuracy. Agents and Actions3, 148–156 (1973).
M. A. Beaven and R. E. Schaff,Study of the relationship of histaminase and diamine oxydase activities in various rat tissues and plasma by sensitiver isotopic assay procedures. Biochem. Pharmacol.24, 979–984 (1975).
J. Kusche, R. Mennigen and I. Rosenthal,Histamine reduces the deamination of putrescine in vitro — but also in vivo? Agents and Actions16, 102–104 (1985).
J. M. Wal, J. C. Meslin, A. Weyer and B. David,Histamine and mast cell distribution in the intestinal wall of the rat: comparison between germ-free and conventional rats. Int. Archs. Allergy appl. Immunol.77, 308–314 (1985).
M. H. Beaver and B. S. Wostmann,Histamine and 5-Hydroxytryptamine in the intestinal tract of germ free animals, animals harbouring one microbial species and conventional animals. Brit. J. Pharmacol.19, 385–393 (1962).
A. M. P. Saavedra-Delgado, S. Turpin and D. D. Metcalfe,Typical and atypical mast-cells of the rat gastrointestinal system: distribution and correlation with tissue histamine. Agents and Actions14, 1–7 (1984).
R. E. Shaff and M. A. Beaven,Turnover and synthesis of diamine oxydase (DAO) in rat tissues. Studies with heparin and cycloheximide. Biochem. Pharmacol.25, 1057–1062 (1976).
S. Henningsson, L. Lundell and E. Rosengren,Comparative study of two isotopic methods determining histamine formation in vitro. Biochem. Pharmacol.23, 2671–2678 (1974).
U. Wingren, L. Enerbäck, H. Ahlman, S. Allenmarck and A. Dahlstrom,Amines of the mucosal mast-cells of the gut in normal and nematode infected rats. Histochemistry77, 145–158 (1983).
M. T. Bauza and D. Lagunoff,Histidine transport by isolated rat peritoneal mast cells. Biochem. Pharmacol.30, 1271–1276 (1981).
K. M. M. Shakir, S. Margolis and S. B. Baylin,Localization of histaminase (diamine oxydase) in rat small intestinal mucosa: site of release by heparin. Biochem. Pharmacol.26, 2343–2347 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huneau, J.F., Tome, D. & Wal, J.M. Histamine content, diamine oxydase and histidine decarboxylase activities along the intestinal tract of the rat. Agents and Actions 28, 231–234 (1989). https://doi.org/10.1007/BF01967407
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01967407