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Intestinal anaphylaxis in the rat: Mediators responsible for the ion transport abnormalities

  • Allery, Histamine and Kinins
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Abstract

Antigen challenge of jejunal epithelium from rats sensitized to egg albumin induces an active Cl− secretory process secondary to release of mucosal mast cell mediators. The present study was designed to define the relative role of these mast cell mediators and the enteric nervous system in the transport abnormalities associated with intestinal anaphylaxis. Net ion transport of stripped jejural tissue from sensitized and sham-treated animals was studied in Ussing chambers. The Cl− secretory response induced by egg albumin during intestinal anaphylaxis was similar to that after addition of 5-hydroxytryptamine (5-HT), histamine, and prostaglandins D2 and E2 to jejunal tissue. Cinanserin, a 5-HT2-receptor antagonist, virtually abolished the response to 5-HT and totally abolished the response to egg albumin. Methysergide, a 5-HT1-receptor antagonist had no effect on either response. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the 5-HT and egg albumin response. Diphenhydramine, an H1-receptor antagonist and cimetidine, an H2-receptor antagonist both significantly inhibited the histamine response but neither altered the response to egg albumin. Atropine, an anticholinergic, and tetrodotoxin, a nerve blocker, did not inhibit the antigen induced anaphylactic response. These results indicate that 5-HT, acting through 5-HT2 receptors is largely responsible for the transport abnormalities seen in intestinal anaphylaxis induced by egg albumin while prostaglandins appear to play a partial role. The findings do not support a role for the enteric nervous system for the egg albumin induced changes in Cl− secretion.

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References

  1. M. H. Perdue, M. Chung and D. G. Gall,Effect of intestinal anaphylaxis on gut function in the rat. Gastroenterology86, 391–397 (1984).

    Google Scholar 

  2. M. H. Perdue and D. G. Gall,Intestinal anaphylaxis in the rat: jejunal response to in vitro antigen exposure. Am. J. Physiol.250, G427-G431 (1986).

    PubMed  Google Scholar 

  3. M. K. Patrick, I. J. Dunn, A. Buret, H. R. P. Miller, J. F. Huntley, S. Gibson and D. G. Gall,Mast cell protease release and mucosal ultrastructure during intestinal anaphylaxis in the rat. Gastroenterology94, 1–9 (1988).

    Google Scholar 

  4. M. H. Perdue and D. G. Gall,Transport abnormalities during intestinal anaphylaxis in the rat: effect of antiallergic agents. J. Allergy Clin. Immunol.76, 498–503 (1985).

    PubMed  Google Scholar 

  5. P. C. Ho, R. A. Lewis, K. F. Austen and R. P. Orange,Comprehensive Immunology, pp. 179–228, Plenum, New York 1979.

    Google Scholar 

  6. J. Hardcastle, P. T. Hardcastle and J. S. Redfern,Action of 5-hydroxytryptamine on intestinal ion transport in the rat. J. Physiol.320, 41–55 (1981).

    PubMed  Google Scholar 

  7. M. H. Perdue and D. G. Gall,Rat jejunal mucosal response to histamine and anti-histamine in vitro. Comparison with antigen-induced changes during intestinal anaphylaxis. Agents and Actions19, 5–9 (1986).

    PubMed  Google Scholar 

  8. M. Field, M. W. Musch, R. L. Miller and E. J. Goetzl,Regulation of epithelial electrolyte transport by metabolites of arachidonic acid. J. Allergy Clin. Immunol.74, 382–385 (1984).

    PubMed  Google Scholar 

  9. D. Branski, M. Eran, P. Sharon, E. Gross-Kieselstein, J. Weidenfeld and S. Freier,Rise of prostanoids in rat small intestinal mucosa following intestinal protein hypersensitivity. Pediatr. Res.21, 414–416 (1987).

    PubMed  Google Scholar 

  10. G. A. Castro, Y. Harari and D. Russell,Mediators of anaphylaxis-induced ion transport changes in small intestine. Am. J. Physiol.253, G540-G548 (1987).

    PubMed  Google Scholar 

  11. H. J. Cooke and H. V. Carey,Pharmacological analysis of 5-hydroxytryptamine actions on guinea-pig ileal mucosa. Eur. J. Pharmacol.111, 329–337 (1985).

    PubMed  Google Scholar 

  12. M. H. Perdue, J. F. Forstner, N. W. Roomi and D. G. Gall,Epithelial response to intestinal anaphylaxis in rats: goblet cell secretion and enterocyte damage. Am. J. Physiol.247, G632-G637 (1984).

    PubMed  Google Scholar 

  13. Z. Ovary,CIOMS Symposium, pp. 259–279. Blackwell Scientific, Oxford 1964.

    Google Scholar 

  14. T. W. Clarkson and S. R. Toole,Measurement of short-circuit current across the intestinal wall of the rat. Am. J. Physiol.207, 415–421 (1964).

    PubMed  Google Scholar 

  15. L.-E. Arvidsson, U. Hacksell and R. A. Glennon,Progress in Drug Research, vol. 30, pp. 365–472, Birkhauser-Verlag, Basel 1986.

    Google Scholar 

  16. J. E. Leyson and P. A. J. Janssen,Receptor binding profile of R41 468, a novel antagonist at 5-HT 2 receptors. Life Science28, 1015–1022 (1981).

    Google Scholar 

  17. J. Hardcastle and P. T. Hardcastle,The secretory actions of histamine in rat small intestine. J. Physiol.388, 521–532 (1987).

    PubMed  Google Scholar 

  18. E. Beubler, K. Bukhave and J. Rask-Madsen,Significance of calcium for the prostaglandin E 2 -mediated secretory response to 5-hydroxytryptamine in the small intestine of the rat in vivo. Gastroenterology90, 1972–1977 (1986).

    PubMed  Google Scholar 

  19. T. Branchek, M. Kates and M. D. Gershon,Enteric receptors for 5-hydroxytryptamine. Brain Res.324, 107–118 (1984).

    PubMed  Google Scholar 

  20. T. S. Gaginella, T. J. Rimele and M. Wietecha,Studies on rat intestinal epithelial cell receptors for serotonin and opiates. J. Physiol. (Lond.)335, 101–111 (1983).

    Google Scholar 

  21. M. Donowitz, N. Asarkof and G. Pike,Calcium dependence of serotonin-induced changes in rabbit ileal electrolyte transport. J. Clin. Invest.66, 341–352 (1980).

    PubMed  Google Scholar 

  22. J. Hardcastle, P. T. Hardcastle and J. M. Noble,The involvement of calcium in the intestinal response to secretagogues in the rat. J. Physiol.355, 465–478 (1984).

    PubMed  Google Scholar 

  23. F. L. Pearce,Calcium and mast cell activation. Br. J. Clin. Pharmc.20, 267S-274S (1985).

    Google Scholar 

  24. A. W. Baird, R. R. A. Coombs, P. McLaughland and A. W. Cuthbert,Immediate hypersensitivity reactions to cow's milk protein in isolated epithelium from ileum of milk-drinking guinea-pigs: comparison with colonic epithelium. Int. Arch. Allergy Appl. Immunol.75, 255–263 (1984).

    PubMed  Google Scholar 

  25. A. W. Cuthbert, P. McLaughlan and R. R. A. Coombs,Immediate hypersensitivity reaction to β-lactoglobulin in the epithelium lining the colon of guinea pigs fed cow's milk. Int. Arch. Allergy Appl. Immunol.72, 34–40 (1983).

    PubMed  Google Scholar 

  26. H. R. P. Miller,The protective mucosal response against gastrointestinal nematodes in ruminants and laboratory animals. Veterinary Immunol. Immunopath.6, 167–259 (1984).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Gastroenterology Research Unit, Health Science Centre, The University of Calgary, 3330 Hospital Drive N. W., T2N 4N1, Calgary, Alberta, Canada

    A. G. Catto-Smith, M. K. Patrick, J. A. Hardin & D. Grant Gall

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  1. A. G. Catto-Smith
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  2. M. K. Patrick
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  3. J. A. Hardin
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  4. D. Grant Gall
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Catto-Smith, A.G., Patrick, M.K., Hardin, J.A. et al. Intestinal anaphylaxis in the rat: Mediators responsible for the ion transport abnormalities. Agents and Actions 28, 185–191 (1989). https://doi.org/10.1007/BF01967399

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  • DOI: https://doi.org/10.1007/BF01967399

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