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Evaluation of immunoblotting for the detection ofToxoplasma gondii immunoglobulin M antibodies

  • C. Verhofstede
  • L. Van Renterghem
  • J. Plum
Notes

Abstract

Immunoblot analysis was used to detect human IgM antibodies toToxoplasma gondii in 20 patients with recent toxoplasmosis, 30 immune individuals, 30 non-immune individuals, and 24 children less then two years old. Analysis of the IgM strips revealed that specific IgM antibodies detectable after a recentToxoplasma gondii infection react with the same antigens as the ‘natural’ antibodies present in the sera of immune and non-immune individuals and in the sera of young children. These data indicate that immunoblotting is not useful as a reference method forToxoplasma gondii IgM detection, and suggest that improvement of the specificity of IgM detection will remain difficult.

Keywords

Internal Medicine Young Child Immunoblot Analysis Reference Method Toxoplasmosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1990

Authors and Affiliations

  • C. Verhofstede
    • 1
  • L. Van Renterghem
    • 1
  • J. Plum
    • 1
  1. 1.Department of Medical MicrobiologyUniversity HospitalGentBelgium

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