Evaluation of immunoblotting for the detection ofToxoplasma gondii immunoglobulin M antibodies

  • C. Verhofstede
  • L. Van Renterghem
  • J. Plum


Immunoblot analysis was used to detect human IgM antibodies toToxoplasma gondii in 20 patients with recent toxoplasmosis, 30 immune individuals, 30 non-immune individuals, and 24 children less then two years old. Analysis of the IgM strips revealed that specific IgM antibodies detectable after a recentToxoplasma gondii infection react with the same antigens as the ‘natural’ antibodies present in the sera of immune and non-immune individuals and in the sera of young children. These data indicate that immunoblotting is not useful as a reference method forToxoplasma gondii IgM detection, and suggest that improvement of the specificity of IgM detection will remain difficult.


Internal Medicine Young Child Immunoblot Analysis Reference Method Toxoplasmosis 
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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1990

Authors and Affiliations

  • C. Verhofstede
    • 1
  • L. Van Renterghem
    • 1
  • J. Plum
    • 1
  1. 1.Department of Medical MicrobiologyUniversity HospitalGentBelgium

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