N-Formylmethionyl-leucyl-phenylalanine: Different releasing effects on human neutrophils and rat mast cells
- 20 Downloads
N-Formylmethionyl-leucyl-phenylalanine (FMLP) is a synthetic chemotactic peptide which induced β-glucuronidase and lysozyme release from human neutrophils treated with cytochalasin B. FMLP-releasing effects were rapid and dose dependent. Unlike other secretagogues of neutrophils (e.g., zymosan and immune complexes), FMLP secretory activity was not modulated by acetylcholine, which by itself did not release lysosomal enzymes from human neutrophils. Isolated rat mast cells did not respond to FMLP, which has been demonstrated to release histamine from human neutrophils. Two markers of rat mast cell secretory granules, histamine and β-glucuronidase, were assayed, but the results were negative for both. In the same experimental conditions, 48/80 released histamine and the enzyme: the ratio of the net percentage release of β-glucuronidase to the net percentage release of histamine was ∼-0.4.
KeywordsPeptide Histamine Acetylcholine Lysozyme Immune Complex
Unable to display preview. Download preview PDF.
- K. Wong andK. Freund,Inhibition of the n-formylmethionyl-leucyl-phenylalanine induced respiratory burst in human neutrophils by adrenergic agonists and prostaglandins of the E series, Can. J. Physiol. Pharmac.59, 915–920 (1981).Google Scholar
- L.J. Ignarro andW.J. George,Hormonal control of lysosomal enzyme release from human neutrophils: elevation of cyclic nucleotide levels by autonomic neurohormones, Proc. natn. Acad. Sci. USA,71, 2027–2031 (1974).Google Scholar
- R.P. Rubin, L.E. Sink andR.J. Freer,On the relationship between formylmethionyl-leucyl-phenylalanine stimulation of arachidonyl phosphatidylinositol turnover and lysosomal enzyme secretion by rabbit neutrophils, Mol. Pharmac.19, 31–37 (1981).Google Scholar
- W.H. Fishman, β-Glucuronidase. InMethods of Enzymatic Analysis, vol. 2, pp. 929–943 (Ed.H.U. Bergmeyer). Verlag Chemie, Academic Press, New York 1974.Google Scholar
- H.U. Bergmeyer andE. Bernt, Lactate dehydrogenase. UV-Assay with pyruvate and NADH. InMethods of Enzymatic Analysis, vol. 2, pp. 574–579 (Ed.H.U. Bergmeyer). Verlag Chemie, Academic Press, New York 1974.Google Scholar
- P. Blandina, R. Fantozzi, P.F. Mannaioni andE. Masini,Characteristics of histamine release evoked by acetylcholine in isolated rat mast cells, J. Physiol, Lond.301, 281–293 (1980).Google Scholar
- R.J. Smith andL.J. Ignarro,Bioregulation of lysosomal enzyme secretion from human neutrophils: roles of guanosine 3′∶5′-monophosphate and calcium in stimulus-secretion coupling, Proc. natn. Acad. Sci. USA72, 108–112 (1975).Google Scholar
- R.B. Zurier, S. Hoffstein andG. Weissmann,Cytochalasin B: effect on lysosomal enzyme release from human leukocytes, Proc. natn. Acad. Sci. USA70, 844–848 (1973).Google Scholar
- B.H. Dulis, M.A. Gordon andI.B. Wilson,Identification of muscarinic binding sites in human neutrophils by direct binding, Mol. Pharmac.15, 28–34 (1979).Google Scholar