Comparison of the effect of PGE2 and somatostatin on histamine stimulated14C-aminopyrine uptake and cyclic AMP formation in isolated rat gastric mucosal cells
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In isolated rat gastric cells somatostatin and PGE2 were compared in respect to their effects on the cAMP system and on the histamine-stimulated H+-production, measured by14C-aminopyrine (14C-AP) uptake. Like PGE2 somatostatin activated adenylate cyclase (AC) for all in non-parietal cells. This effect on AC declined in cell fractions with increasing number of parietal cells. Activation of AC or elevation of cellular cAMP and uptake of14C-AP in response to histamine were inhibited by 10−9 to 10−5 mol/l PGE2 and somatostatin. The results indicate remarkable similarity between somatostatin and PGE2: both activate a non-parietal cell AC and both inhibit H+-production, likely by interfering at the histamine sensitive AC of the parietal cell.
KeywordsHistamine PGE2 Adenylate Cyclase Parietal Cell Mucosal Cell
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