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, Volume 26, Issue 3–4, pp 287–291 | Cite as

Lipopolysaccharide stimulates alveolar macrophage adherencein vivo andin vitro

  • J. Edelman
  • C. Cardozo
  • M. Lesser
Allergy, Histamine and Kinins

Abstract

The number of alveolar macrophages (AM) obtained from rats by bronchoalveolar lavage (BAL) decreases significantly within two hours after infusion ofE. coli lipopolysaccharide (LPS). To investigate the mechanism of this phenomenon, we evaluated adherence properties of AM obatined from rats 4 hours after intravenous injection ofE. coli LPS (3 mg/kg) or normal saline (NS). Significantly fewer AM were present in BAL samples from LPS-treated rats (4.09×106) compared to NS-treated rats (7.55×106). When a 17 mM lidocaine solution (LDC) was used for lavage, the number of AM in BAL samples from LPS-treated rats increased significantly (7.38×106). Adherence of AM from LPS-treated rats to rotating plastic tubes was significantly greater than that of AM from NS-treated rats. Incubation of normal AM with LPS (5 or 15 μg/ml) in serum-free media increased AM adherence to plastic. LPS-induced adherence was significantly greater at the higher LPS dose. Thus, the decreased number of AM obtained by BAL of LPS-treated rats appears to be due to increased adherence of AM to alveolar structures. This phenomenon may be mediated by direct effects of LPS on AM.

Keywords

Lidocaine Intravenous Injection Alveolar Macrophage Bronchoalveolar Lavage Plastic Tube 

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Copyright information

© Birkhäuser Verlag 1989

Authors and Affiliations

  • J. Edelman
    • 1
    • 4
    • 5
  • C. Cardozo
    • 2
    • 4
    • 5
  • M. Lesser
    • 3
    • 4
    • 5
  1. 1.Pulmonary DivisionThe Mount Sinai Medical CenterNew York
  2. 2.Pulmonary DivisionThe Mount Sinai Medical CenterNew YorkUSA
  3. 3.Bronx Veterans Administration medical CenterPulmonary SectionNew YorkUSA
  4. 4.Veterans Administration Medical CenterBronx
  5. 5.the Department of MedicineThe Mount Sinai Medical CenterNew YorkUSA

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