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Latex agglutination for rapid identification of methicillin-resistantStaphylococcus aureus recovered from selective media

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Abstract

The accuracy of combining latex agglutination with selective media for the identification of methicillin-resistantStaphylococcus aureus (MRSA) was determined. Test strains were identified by latex agglutination on blood agar, the heat-stable thermonuclease test and broth microdilution MICs of oxacillin and included 97 MRSA, 56 methicillin-susceptibleStaphylococcus aureus, 52 methicillin resistant, and 49 methicillin-susceptibleStaphylococcus species. Isolates were grown on trypticase-soy agar with 5 % sheep red blood cells (TSAB), Mueller-Hinton agar (MHA), mannitol-salt agar (MSA), and four media designed for the selective growth of MRSA: TSAB with clindamycin and gentamicin, MHA with oxacillin, MSA with oxacillin, and lipovitellin-salt-mannitol agar (LVSM) with 1 µg oxacillin disks applied. The mean sensitivity, specificity, and positive predictive value for the combination of latex agglutination with selective media for the identification of MRSA was 96 %, 99 % and 98 % respectively.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of Texas Medical School, PO Box 20708, 77030, Houston, Texas, USA

    A. R. Wanger, D. G. Moore & M. T. LaRocco

Authors
  1. A. R. Wanger
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  2. D. G. Moore
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  3. M. T. LaRocco
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Wanger, A.R., Moore, D.G. & LaRocco, M.T. Latex agglutination for rapid identification of methicillin-resistantStaphylococcus aureus recovered from selective media. Eur. J. Clin. Microbiol. Infect. Dis. 10, 564–567 (1991). https://doi.org/10.1007/BF01967274

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  • DOI: https://doi.org/10.1007/BF01967274

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