Abstract
The E test, a new technique for measuring MICs of antimicrobial agents with the ease of disc diffusion tests, was evaluated in testing the susceptibility of 94 clinical isolates ofPseudomonas aeruginosa to tobramycin. The use of the E test was found acceptable; 93 % of the MIC results were within one log2 dilution step and 100 % were within two log2 dilution steps when the MICs obtained by the E test were compared to those obtained by the conventional agar dilution method. When the E test was compared to the broth microdilution method the corresponding figures were 84 % and 100 %, respectively.
Similar content being viewed by others
References
Washington JA, Snyder RJ, Kohner PC, Wiltse CG, Ilstrup DM, McCall JT: Effect of cation content of agar on the activity of gentamicin, tobramycin, and amikacin againstPseudomonas aeruginosa. Journal of Infectious Diseases 1978, 137: 103–111.
Casillas E, Kenny MA, Minshew BH, Schoenknecht FD: Effect of ionized calcium and soluble magnesium on the predictability of the performance of Mueller-Hinton agar susceptibility testing ofPseudomonas aeruginosa with gentamicin. Antimicrobial Agents and Chemotherapy 1981, 19: 987–992.
National Committee for Clinical Laboratory Standards: Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Tentative standard M7-T. Volume 3. NCCLS, Villanova, PA, 1982.
Brown DFJ, Brown L: Evaluation of the E test, a novel method of quantifying antimicrobial activity. Journal of Antimicrobial Chemotherapy 1991, 27: 185–190.
Baker CN, Stocker SA, Culver DH, Thornsberry C: Comparison of the E test to agar dilution, broth microdilution and agar diffusion susceptibility testing techniques by using a special challenge set of bacteria. Journal of Clinical Microbiology 1991, 29: 533–538.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rautelin, H., Renkonen, I. & Renkonen, O.V. Evaluation of the E test in testing susceptibility ofPseudomonas aeruginosa to tobramycin. Eur. J. Clin. Microbiol. Infect. Dis. 11, 177–180 (1992). https://doi.org/10.1007/BF01967074
Issue Date:
DOI: https://doi.org/10.1007/BF01967074