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, Volume 15, Issue 1–2, pp 87–89 | Cite as

Radioimmunological determination of prostaglandin D2 after conversion to a stable degradation product

  • Klaus Lange
  • Bernhard A. Peskar
Inflammation and Anti-Inflammatories: Miscelaneous Studies

Abstract

Prostaglandin (PG)D2 was found to be unstable under various conditions. A radioimmunoassay for a stable degradation product of PGD2 formed by incubation at alkaline pH was developed. The radioimmunoassay is considerably more specific and sensitive than a comparable radioimmunoassay for PGD2. Using the radioimmunoassay for the stable degradation product release of large amounts of PGD2 from anaphylactic guinea-pig hearts was observed.

Keywords

Prostaglandin Degradation Product Product Release Stable Degradation Radioimmunological Determination 

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References

  1. [1]
    R.J. Flower, E.A. Harvey andW.P. Kingston,Inflammatory effects of prostaglandin D 2 in rat and human skin, Br. J. Pharmac.56, 229–233 (1976).Google Scholar
  2. [2]
    J.B. Smith, M.J. Silver, C.M. Ingerman andJ.J. Kocsis,Prostaglandin D 2 inhibits the aggregation of human platelets, Thrombosis Res.5, 291–299 (1974).Google Scholar
  3. [3]
    E.E. Nishizawa, W.L. Miller, R.R. Gorman, G.L. Bundy, J. Svensson andM. Hamberg,Prostaglandin D 2 as a potential antithrombotic agent, Prostaglandins9, 109–121 (1975).PubMedGoogle Scholar
  4. [4]
    M. Fukushima, T. Kato, R. Ueda, K. Ota, S. Narumiya andO. Hayaishi,Prostaglandin D 2,a potential antineoplastic agent, Biochem. biophys. Res. Commun.105, 956–964 (1982).PubMedGoogle Scholar
  5. [5]
    M.A. Wasserman, D.W. DuCharme, R.L. Griffin, G.L. DeGraaf andF.G. Robinson,Bronchopulmonary and cardiovascular effects of prostaglandin D 2 in the dog, Prostaglandins13, 255–269 (1977).PubMedGoogle Scholar
  6. [6]
    H. Anhut, B.A. Peskar, W. Wachter, B. Grãbling andB.M. Peskar,Radioimmunological determination of prostaglandin D 2 synthesis in human thrombocytes, Experientia34, 1494–1496 (1978).PubMedGoogle Scholar
  7. [7]
    L. Levine, I. Alam andJ.J. Langone,The use of immobilized ligands and ( 125 I)protein A for immunoassays of thromboxane B 2 , prostaglandin D> 2 , 13,14-dihydro-prostaglandin E 2 , 5,6-dihydro-prostaglandin I 2 , 6-keto-prostaglandin F , 15-hydroxy-9α,11α(epoxymethano) prosta-5,13-dienoic acid and 15-hydroxy-11α,9α(epoxymethano) prosta-5,13-dienoic acid, Prostaglandins Med.2, 177–189 (1979).PubMedGoogle Scholar
  8. [8]
    R.J. Gaudet, I. Alam, andL. Levine,Accumulation of cyclooxygenase products of arachidonic acid metabolism in gerbil brain during reperfusion after bilateral common carotid artery occlusion, J. Neurochem.35, 653–658 (1980).PubMedGoogle Scholar
  9. [9]
    S. Narumiya, T. Ogorochi, K. Nakao andO. Hayaishi,Prostaglandin D 2 in rat brain, spinal cord and pituitary: basal level and regional distribution, Life Sci.31, 2093–2103 (1982).PubMedGoogle Scholar
  10. [10]
    H. Anhut, B.A. Peskar andW. Bernauer,Release of 15-keto-13,14-dihydro-thromboxane B 2 and prostaglandin D 2 during anaphylaxis as measured by radioimmunoassay, Naunyn-Schmiedebergs Arch. Pharmak.305, 247–252 (1978).Google Scholar
  11. [11]
    H.C. Kiefer, C.R. Johnson andK.L. Arora,Colorimetric identification of prostaglandins in subnanomole amounts, Anal. Biochem.68, 336–340 (1975).PubMedGoogle Scholar
  12. [12]
    B.F. Erlanger, F. Borek, S.M. Beiser andS. Liebermann,Steroid-protein conjugates. I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and cortisone, J. biol. Chem.228, 713–727 (1957).PubMedGoogle Scholar
  13. [13]
    E. Granström andH. Kindahl, Radioimmunoassay of prostaglandins and thromboxanes. InAdv. in Prostaglandin and Thromboxane Research, Vol. 5, pp. 119–210 (Ed.J.C. Frölich), Raven Press, New York 1978.Google Scholar
  14. [14]
    E. Granström, M. Hamberg, G. Hansson andH. Kindahl,Chemical instability of 15-keto-13,14-dihydro-PGE 2 : the reason for low assay reliability, Prostaglandins19, 933–957 (1980).PubMedGoogle Scholar
  15. [15]
    F.A. Fitzpatrick, R. Aguirre, J.E. Pike andF.H. Lincoln,The stability of 13,14-dihydro-15-keto-PGE 2, Prostaglandins19, 917–931 (1980).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1984

Authors and Affiliations

  • Klaus Lange
    • 1
  • Bernhard A. Peskar
    • 1
  1. 1.Institute of Pharmacology and ToxicologyRuhr-University BochumBochum 1FRG

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