Radioimmunological determination of prostaglandin D2 after conversion to a stable degradation product
Prostaglandin (PG)D2 was found to be unstable under various conditions. A radioimmunoassay for a stable degradation product of PGD2 formed by incubation at alkaline pH was developed. The radioimmunoassay is considerably more specific and sensitive than a comparable radioimmunoassay for PGD2. Using the radioimmunoassay for the stable degradation product release of large amounts of PGD2 from anaphylactic guinea-pig hearts was observed.
KeywordsProstaglandin Degradation Product Product Release Stable Degradation Radioimmunological Determination
Unable to display preview. Download preview PDF.
- R.J. Flower, E.A. Harvey andW.P. Kingston,Inflammatory effects of prostaglandin D 2 in rat and human skin, Br. J. Pharmac.56, 229–233 (1976).Google Scholar
- J.B. Smith, M.J. Silver, C.M. Ingerman andJ.J. Kocsis,Prostaglandin D 2 inhibits the aggregation of human platelets, Thrombosis Res.5, 291–299 (1974).Google Scholar
- L. Levine, I. Alam andJ.J. Langone,The use of immobilized ligands and ( 125 I)protein A for immunoassays of thromboxane B 2 , prostaglandin D> 2 , 13,14-dihydro-prostaglandin E 2 , 5,6-dihydro-prostaglandin I 2 , 6-keto-prostaglandin F 1α , 15-hydroxy-9α,11α(epoxymethano) prosta-5,13-dienoic acid and 15-hydroxy-11α,9α(epoxymethano) prosta-5,13-dienoic acid, Prostaglandins Med.2, 177–189 (1979).PubMedGoogle Scholar
- H. Anhut, B.A. Peskar andW. Bernauer,Release of 15-keto-13,14-dihydro-thromboxane B 2 and prostaglandin D 2 during anaphylaxis as measured by radioimmunoassay, Naunyn-Schmiedebergs Arch. Pharmak.305, 247–252 (1978).Google Scholar
- E. Granström andH. Kindahl, Radioimmunoassay of prostaglandins and thromboxanes. InAdv. in Prostaglandin and Thromboxane Research, Vol. 5, pp. 119–210 (Ed.J.C. Frölich), Raven Press, New York 1978.Google Scholar