Skip to main content
SpringerLink
Log in

Rabbit monoarticular arthritis as a model for intra-articular drug injections. The local action of administered cortisol and alpha-1 proteinase inhibitor

  • Immunosuppression and Inflammation
  • Editorial
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Monoarticular arthritis in the rabbit has been used to study the effect of intra-articular administration of cortisol-21-phosphate and alpha-1-proteinase inhibitor. The preparations were administered both separately and in combination. All treatments improved parameters associated with joint biochemistry and histopathology, but the greatest effect was found when steroid was combined with antiproteinase. Cortisol-21-phosphate had both an anti-inflammatory and anti-arthritic action, whereas alpha-1-proteinase inhibitor showed little anti-inflammatory action but had some anti-arthritic effect. Alpha-1-proteinase inhibitor had no anti-inflammatory action against carrageenan induced oedema in the rat, but was anti-arthritic against adjuvant induced arthritis in the rat where it reduced both primary and secondary arthritis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. D.C. Dumonde andL.E. Glynn,The production of arthritis in rabbits by an immunological reaction to fibrin, Br. J. exp. Path.43, 373–383 (1962).

    Google Scholar 

  2. L.E. Glynn,The chronicity of inflammation and its significance in rheumatoid arthritis, Ann. rheum. Dis.27, 105–121 (1968).

    PubMed  Google Scholar 

  3. L.E. Glynn,Aetiology of rheumatoid arthritis with regard to its chronicity, Ann. rheum. Dis.28, Suppl. 6, 3–4 (1969).

    PubMed  Google Scholar 

  4. L.M. Hunneyball,Some further effects of prednisolone and triamcinolone hexacetonide in experimental arthritis in rabbits, Agents and Actions11, (5), 490–498 (1981).

    PubMed  Google Scholar 

  5. M. Corbett, M.H. Serbert, C. Hacking andS. Webb,Comparison between local injections of silicon oil and hydrocortisone acetate in chronic arthritis, Brit. Med. J.1, 24–25 (1970).

    PubMed  Google Scholar 

  6. J. Lieberman andW. Kaneshiro,Inhibition of leukocytic elastase from purulent sputum by alpha-1-antitrypsin, J. Lab. clin. Med.80, 80–101 (1972).

    Google Scholar 

  7. F. Kueppers andL.F. Black,Alpha-1-antitrypsin and its deficiency, Am. Rev. resp. Dis.110, (2) 176–194 (1974).

    PubMed  Google Scholar 

  8. D. Brackertz,Proteinase inhibitoren bei rheumatischen erkrankugen, Dt. med. Wschr.103, (46) 1844–1849 (1978).

    Google Scholar 

  9. A. Rimon, Y., Shamash andB. Shapiro,The plasmin inhibitor of human plasma. VI. Its action on plasmin, trypsin, chymotrypsin and thrombin, J. biol. Chem241, 5102–5107 (1966).

    PubMed  Google Scholar 

  10. E.D. Harris, D.R. Di Bona andS.M. Krane,Collagenases in human synovial fluid, J. clin. Invest.48, 2104–2113 (1969).

    PubMed  Google Scholar 

  11. G. Shtacher, R. Maayan andG.A.D. Feinstein,Protease inhibitors in human synovial fluid, Biochim. biophys. Acta303, 138–147 (1973).

    PubMed  Google Scholar 

  12. C.G. Cochrane,Plasma proteins and inflammatory disease, Pharmac. Rev.34 (1) 39–41 (1982).

    Google Scholar 

  13. C.A. Winter, E.A. Risley andG.W. Nuss,Carrageenin induced oedema in the hind paw of the rat, an assay for anti-inflammatory drugs, Proc. Soc. exp. Biol. Med.11, 544–547 (1962).

    Google Scholar 

  14. D.P. Parrott andD.A. Lewis,Protease and antiprotease levels in blood in arthritic rats, Ann. rheum. Dis.36, 166–169 (1977).

    PubMed  Google Scholar 

  15. C.B. Laurell, J. Pierce, U. Persson andE. Thuun,Purification of alpha-1-antitrypsin from plasma through thiol-disulphide exchange, Eur. J. Biochem.57, 107–113 (1975).

    Article  PubMed  Google Scholar 

  16. B.B. Newbould,Chemotherapy of arthritis induced in rats by mycobacterial adjuvant, Brit. J. Pharmac.21, 1–10 (1963).

    Google Scholar 

  17. A.A. Dietz, H.M. Rubenstein andL. Hodges,Measurement of alpha-1-antitrypsin in serum by immunodiffusion and by enzymatic assay. Clin. Chem.20, 396–399 (1974).

    PubMed  Google Scholar 

  18. O.H. Lowry, N.J. Rosebrough, A.L. Farr andR.J. Randall,Protein measurement with the Folin phenol reagent, J. biol. Chem.193, 265–275 (1951).

    PubMed  Google Scholar 

  19. A.M. Symons, D.A. Lewis andR.J. Ancill,Stabilising action of anti-inflammatory steroids on lysosomes, Biochem. Pharmac.18, 2561–2582 (1969).

    Article  Google Scholar 

  20. H. Rinderknecht, M.C. Geokas, P. Silverman andJ.B. Haverback,A new ultrasensitive method for the determination of proteolytic activity, Clinica chim. Acta21, 197–203 (1969).

    Article  Google Scholar 

  21. E.D. Maguire andR.B. Wallis,The role of bacteria contamination in the isolation of apparent anti-inflammatory factors from rabbit anti-lymphocytic serum, Brit. J. Pharmac.59, 261–487 (1977).

    Google Scholar 

  22. D.C. Atkinson andR. Hicks,Relationship between the anti-inflammatory and irritant properties of inflammatory exudate, Br. J. Pharmac.41, 480–487 (1977).

    Google Scholar 

  23. A. Blackman andH. Radziwonik,The effect of drugs in established rabbit monoarticular arthritis, Agents and Actions7, 473–480 (1977).

    Google Scholar 

  24. B. Davies,Effects of prednisolone in an experimental model of arthritis in the rabbit, Ann. rheum. Dis.30, 509–521 (1971).

    PubMed  Google Scholar 

  25. J.M. Dayer, J. Breard, L. Chess andS.M. Krane,Participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and prostaglandin release by rheumatoid synovial cells, J. clin. Invest.64, 1386–1392 (1979).

    PubMed  Google Scholar 

  26. M.K.B. McGuire, J.E. Meats, N.M. Ebsworth, R.G.G. Russell, G. Murphy andJ.J. Reynolds, Effects of corticosteroids on cellular interactions in human joint tissues in culture. InSeminars in Arthritis and Rheumatism, vol. XI, suppl. 1 (Ed.D.S. Howell andJ.H. Talbot), pp. 138–139. Grune and Stratton. Inc., New York 1981.

    Google Scholar 

  27. M.K. McGuire, G. Murphy, J.J. Reynolds andR.G.G. Russell,The production of collagen and collagenase inhibitor by normal and rheumatoid synovium: the effects of hydrocortisone and indomethacine, Clin. Sci.61, 703–710 (1981).

    PubMed  Google Scholar 

  28. R.J. Flower andG.J. Blackwell,Anti-inflammatory steroids induce biosynthesis of a phospholipase A 2 inhibitor which prevents prostaglandin generation, Nature278,456–459 (1979).

    PubMed  Google Scholar 

  29. F. Hirata, R. Del Carmine, C.A. Nelson, J. Axelrod, E. Schiffmann, A. Warabi, A.L. De Blas, M. Nirenberg, V. Manganiello, M. Vaughan, S. Kumagi, I. Green, J.L. Decker andA.D. Steinberg,Presence of autoantibody for phospholipase inhibitory protein, lipomodulin, in patients with rheumatic diseases, Proc. Natn Acad Sci USA (1981).

  30. V. Wright andR. Amos,Do drugs change the course of rheumatoid arthritis?, Br. Med. J.280, 964–966 (1980).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pharmacological Laboratories, Department of Pharmaceutical Sciences, University of Aston in Birmingham, Gosta Green, B4 7ET, Birmingham, UK

    Eva Pitt & D. A. Lewis

Authors
  1. Eva Pitt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. A. Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pitt, E., Lewis, D.A. Rabbit monoarticular arthritis as a model for intra-articular drug injections. The local action of administered cortisol and alpha-1 proteinase inhibitor. Agents and Actions 15, 541–548 (1984). https://doi.org/10.1007/BF01966771

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01966771

Search

Navigation