Agents and Actions

, Volume 17, Issue 1, pp 80–83 | Cite as

In vivo modulation of leukotaxis by non-steroidal anti-inflammatory drugs

  • Takao Shimanuki
  • Robert M. Nakamura
  • Gere S. diZerega
Immunosuppression and Inflammation

Abstract

Since non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for inhibition of inflammation, anin vivo assay for leukotaxis would be of use in comparing the biological activity effects of the agents. Here, the effects of 4 different NSAIDs onin vivo leukocyte accumulation was determined by quantitatingN-formyl-methionyl-leucylphenylalanine induced leukotaxis in the rabbit anterior eye chamber. New Zealand white female rabbits were treated for three days with the following regimens: ibuprofen or aspirin, 0.1, 1.0, 10.0 or 100.0 mg/kg/day, indomethacin or flurbiprofen, 0.01, 0.1, 1.0, or 10.0 mg/kg/day. Indomethacin and flurbiprofen significantly reduced leukotaxis in a dose of 10.0 mg/kg/day. Aspirin was associated with a weak inhibition of leukotaxis. Ibuprofen had biphasic effects, 1.0 mg/kg/day potentiated and 10 mg/kg/day inhibited leukotaxis, whereas leukocyte accumulation was uneffected by a high dose (100.0 mg/kg/day). These results suggest that modulation of leukotaxis by NSAIDs may reflect a differential dose-response sensitivity of lipoxygenase and cycloxygenase pathways.

Keywords

Aspirin Biological Activity Indomethacin Ibuprofen Activity Effect 

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Copyright information

© Birkhäuser Verlag 1985

Authors and Affiliations

  • Takao Shimanuki
    • 2
  • Robert M. Nakamura
    • 1
  • Gere S. diZerega
    • 1
  1. 1.Livingston Reproductive Biology Laboratory, Department of Obstetrics and GynecologyUniversity of Southern California School of MedicineLos Angeles
  2. 2.The Second Department of SurgeryYamagata School of MedicineYamagataJapan

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