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, Volume 14, Issue 2, pp 153–156 | Cite as

Inhibition of rat mast cell degranulation by verapamil

  • A. Legrand
  • J. Cerrina
  • C. Bonne
  • A. Lockhart
  • J. Benveniste
Histamine and Kinins

Abstract

Calcium antagonists, e.g. verapamil, prevent exercise-induced asthma. This protective effect may proceed from inhibition of contraction of bronchial smooth muscle, release of mediators by primary effector cells, e.g. mast cells, or both. Therefore, we studied the inhibitory effect of increasing concentrations of verapamil on bothin vitro antigen-induced degranulation and ionophore A23187-induced release of labelled serotonin by rat peritoneal mast cells. There was a dose-dependent inhibition by verapamil of both ovalbumin-induced degranulation of mast cells passively sensitized by incubation with mice IgE-rich serum and ionophore-induced release of tritiated serotonin by mast cells previously incubated with (3H)-5HT; the 50% inhibiting concentration was 1·4×10−4 mol l−1 and 5·2×10−5 mol l−1, respectively. An attractive explanation of our results is that verapamil inhibits the antigen-induced release of mediators by mast cells through its calcium antagonist effect. Our results also suggest that the preventing effect of calcium antagonists on asthma may be multi-factorial since other authors have clearly shown that these drugs inhibit contraction of guinea-pig tracheal smooth musclein vitro.

Keywords

Asthma Serotonin Mast Cell Verapamil Calcium Antagonist 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser-Verlag 1984

Authors and Affiliations

  • A. Legrand
    • 1
    • 3
  • J. Cerrina
    • 2
  • C. Bonne
    • 3
  • A. Lockhart
    • 1
  • J. Benveniste
    • 4
  1. 1.Laboratoire d'explorations fonctionnellesHôpital Antoine BéclèreClamartFrance
  2. 2.Service de PneumologieHôpital Antoine BéclèreClamartFrance
  3. 3.INSERM U 235ParisFrance
  4. 4.INSERM U 200ClamartFrance

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