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, Volume 21, Issue 3–4, pp 278–280 | Cite as

Taxol inhibits N-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced human neutrophil polarization and H2O2 production while decreasing [3H]FMLP binding

  • M. A. Iannone
  • G. Wolberg
  • R. Reynolds-Vaughn
  • T. P. Zimmerman
Antiinflammatory/Antiarthritic Agents

Abstract

We have studied the effect of taxol on two N-formyl-methionyl-leucyl-phenylalanine (FMLP)-induced neutrophil functions and the possible mechanism by which it inhibits these functions. Taxol inhibited FMLP-induced human neutrophil polarization (a characteristic change in neutrophil shape in response to a chemotactic stimulus) and H2O2 generation. Taxol also decreased the specific binding of [3H]FMLP to human neutrophils at 4°C. The decreased binding of FMLP to its receptor may be responsible for the inhibition by taxol of FMLP-induced polarization and H2O2 generation.

Keywords

H2O2 Specific Binding Taxol Human Neutrophil Characteristic Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    P. B. Schiff, J. Fant and S. B. Horwitz,Promotion of microtubule assembly in vitro by taxol. Nature, Lond.227, 665–667 (1979).Google Scholar
  2. [2]
    P. B. Schiff and S. B. Horwitz,Taxol stabilizes microtubules in mouse fibroblast cells. Proc. natn. Acad. Sci. USA77, 1561–1565 (1980).Google Scholar
  3. [3]
    J. A. Cuthbert and J. W. Shay,Microtubules and lymphocyte responses: effect of colchicine and taxol on mitogen-induced human lymphocyte activation and proliferation. J. cell. Physiol.116, 127–134 (1983).CrossRefPubMedGoogle Scholar
  4. [4]
    R. L. Roberts, J. N. Nath, M. M. Friedman and J. I. Gallin,Effects of taxol on human neutrophils. J. Immunol.129, 2134–2141 (1982).PubMedGoogle Scholar
  5. [5]
    A. Ferrante and Y. H. Thong,A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the hypaqueficoll technique. J. Immunol. Methods,24, 389–393 (1978).CrossRefPubMedGoogle Scholar
  6. [6]
    C. W. Smith, J. C. Hollers, R. A. Patrick and C. Hassett,Motility and adhesiveness in human neutrophils: effects of chemotactic factors. J. clin. Invest.63, 221–229 (1979).PubMedGoogle Scholar
  7. [7]
    W. Ruch, P. H. Cooper and M. Baggiolini,Assay of H 2O2 production by macrophages and neutrophils with homovanillic acid and horse-radish peroxidase. J. Immunol. Methods63, 347–357 (1983).PubMedGoogle Scholar
  8. [8]
    J. T. Helwig and K. A. Council (Eds.)SAS user's guide. SAS Institute, Raleigh, N. C. 1979.Google Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • M. A. Iannone
    • 1
  • G. Wolberg
    • 1
  • R. Reynolds-Vaughn
    • 1
  • T. P. Zimmerman
    • 1
  1. 1.Wellcome Research LaboratoriesResearch Triangle ParkUSA

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