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Involvement of reactive oxygen species in the microsomal S-oxidation of thiobenzamide

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Superoxide dismutase, catalase and methional proved capable of inhibiting the microsomal oxidation of thiobenzamide, which is most probably catalyzed by the flavin-containing monooxygenase. This indicates that excited oxygen species (e. g.·O −2 , H2O2, ·OH) are involved in the catalytic cycle of this enzymatic reaction. CO, which inhibits the cytochrome P-450-dependent oxygen radical formation, had no effect on the oxidation reaction, suggesting that the source of the reactive oxygen species is not the microsomal mixed-function oxidase.

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  1. Institut für Toxikologie der Medizinischen Hochschule Lübeck, Ratzeburger Allee 160, D-2400, Lübeck, Federal Republic of Germany

    M. Younes

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  1. M. Younes
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Younes, M. Involvement of reactive oxygen species in the microsomal S-oxidation of thiobenzamide. Experientia 41, 479–481 (1985). https://doi.org/10.1007/BF01966157

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