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Experientia

, Volume 36, Issue 9, pp 1035–1037 | Cite as

Continuous optical assay of sucrase and other glucosidases

  • P. Vanni
  • G. M. Hanozet
Specialia

Summary

A continuous optical method for the assay of glucose-releasing hydrolases is reported. Particular emphasis is given to the assay of purified sucrase from rabbit small intestine. The procedure requires glucose dehydrogenase and mutarotase. In the presence of the latter enzyme, the initial lag is substantially shortened when glucose is released asa-anomer. Under the test conditions used, the method shows a good proportionality up to an activity of 0.2 units/3 ml and may also be applied for measuring the activity in crude homogenates.

Keywords

Glucose Enzyme Small Intestine Hydrolase Optical Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 4.
    A. Dahlqvist, Analyt. Biochem.7, 18 (1964).Google Scholar
  2. 5.
    A. Dahlqvist, Analyt. Biochem.22, 99 (1968).PubMedGoogle Scholar
  3. 6.
    G. Semenza and A.K. von Balthazar, Eur. J. Biochem.41, 149 (1974).PubMedGoogle Scholar
  4. 7.
    M. Kessler, O. Acuto, C. Storelli, H. Murer, M. Müller and G. Semenza, Biochim. biophys. Acta506, 136 (1978).PubMedGoogle Scholar
  5. 8.
    S. Messer and A. Dahlqvist, Analyt. Biochem.14, 376 (1966).PubMedGoogle Scholar
  6. 9.
    J. Larner and C.M. McNickle, J. biol. Chem.215, 723 (1955).PubMedGoogle Scholar
  7. 10.
    D. Banauch, W. Brümmer, W. Ebeling, H. Metz, H. Lang, K. Leybold and W. Rick, Z. klin. Chem. Biochem.13, 101 (1975).Google Scholar
  8. 11.
    O.H. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall, J. biol. Chem.193, 265 (1951)PubMedGoogle Scholar
  9. 12.
    A. Cogoli, H. Mosimann, C. Vock, A.K. von Balthazar and G. Semenza, Eur. J. Biochem.30, 7 (1972).PubMedGoogle Scholar
  10. 13.
    H. Sigrist, P. Rommer and G. Semenza, Biochim. biophys. Acta406, 433 (1975).PubMedGoogle Scholar
  11. 14.
    H.U. Bergmeyer, E. Bernt, F. Schmidt and H. Stork, in: Methods of Enzymatic Analysis, vol. 3, p. 1196, Ed. H.U. Bergmeyer. Verlag Chemie, Weinheim 1974.Google Scholar
  12. 15.
    G. Semenza, H. Ch. Curtius, O. Ranhardt, P. Hore and M. Müller, Carbohydr. Res.10, 417 (1969).Google Scholar
  13. 16.
    J. Kolinskà and G. Semenza, Biochim. biophys. Acta146, 181 (1967).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • P. Vanni
    • 1
  • G. M. Hanozet
    • 1
  1. 1.Laboratorium für BiochemieEidgenössische Technische Hochschule ZürichZürichSwitzerland

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