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The quantitative assessment of topical irritancy and its application to unphysiological pH-values

  • Models in Toxicology
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Abstract

Injection of aqueous solutions of topically irritant substances intracutaneously into the skin of juvenile white mice creates a persisting oedema which can be assessed by the number of wrinkles formed upon reefing the skin at the site of injection. An algorithm is presented which transforms the number of wrinkles recorded just before as well as 2.5 h and 6 h after injection into the intensity of the oedematous reaction. The results obtained with a collective of about 10 mice per solution to be tested are reliable and reproducible; they conform to dose-response relations and hence can serve for quantifying local irritancy. The method is used to investigate the effect of pH, and a general model is developed describing the local irritancy of unphysiological pH-values.

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Authors and Affiliations

  1. Department of Pharmacology, Biozentrum, University of Basel, Basel, Switzerland

    D. Walz & K. E. Bucher

Authors
  1. D. Walz
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  2. K. E. Bucher
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Additional information

This work was encouraged and financially supported by the Swiss Federal Office of Health, Section Toxicology.

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Walz, D., Bucher, K.E. The quantitative assessment of topical irritancy and its application to unphysiological pH-values. Agents and Actions 12, 552–565 (1982). https://doi.org/10.1007/BF01965942

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  • DOI: https://doi.org/10.1007/BF01965942

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