The effect of protease inhibitors on the immune response in mice
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The effect of protease inhibitors on the immune response in mice was investigated. Soybean inhibitor, aprotinin, α-antitrypsin and ovomucoid (1 mg) diminished significantly the direct plaque forming cell response per spleen 3 days after immunization with 108 sheep erythrocytes (SRBC), when given at the same time as the antigen. With α-antitrypsin, the effect was not significant 4 days after immunization. With soybean inhibitor, no significant suppressive effect was seen, when the inhibitor was given either 24 hours before or after the antigen. Both aprotinin and ovomucoid diminished a secondary response to SRBC, limited to the indirect plaque forming cells. Soybean inhibitor had an equivocal effect on the secondary response. A suppressive effect of soybean inhibitor, but not ovomucoid was seen using KLH-FITC or LPS-FITC as antigens. Neither soybean inhibitor nor aprotinin, had an effect on the induction of delayed-type skin reactivity to, oxazolone or FITC.
KeywordsImmune Response Protease Inhibitor Cell Response FITC Suppressive Effect
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- F.L. Adler,Competition of antigens, Prog., Allergy8, 41 (1964).Google Scholar
- J.P. Dessaint, S.P. Katz andB.H. Waksman,Catheptic carboxypeptidase B as major component in “T cell-activating factor” of macrophages, J. Immunopharm.1, 399 (1979).Google Scholar
- D.W. Dresser andH.H. Wortis,Localized haemolysis in gel. InHandbook of Experimental Immunology, p. 1054. (Ed.D.M. Weir). Blackwell Scientific Publications, Oxford 1967.Google Scholar
- L.B. Epstein, M.J. Cline andT.C. Merigan,The interaction of human macrophages and lymphocytes in the phytohemagglutinin-stimulated production of interferon, J. Clin. Invest.50, 744 (1971).Google Scholar
- E.J. Holborow andG.D. Johnson,Immunofluorescence. InHandbook of Experimental Immunology, p. 570. (Ed.D.M. Weir). Blackwell Scientific Publications, Oxford 1967.Google Scholar
- E. Reich, D.B. Rifkin andE. Shaw,Proteases and biological control, Cold Spring Harbor Conferences on Cell Proliferation, vol. 2, Cold Spring Harbor Laboratory (1975).Google Scholar
- H. Umezawa, T. Aoyagi, H. Suda, M. Hamada andT. Takenchi,Bestatin, an inhibitor of aminopeptidase B, produced by actinomycetes, J. Antibiotics29, 97 (1976).Google Scholar