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Vascular permeability changes by proteinase inhibitors in carrageenin-induced inflammation in rats

  • Immunosuppression and Inflammation
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Abstract

The effect of proteinase inhibitors such as TLCK, TPCK and leupeptin on vascular permeability was investigated in the carrageenin-air-pouch inflammation in rats. When each inhibitor was injected into the air-pouch immediately after carrageenin injection, TLCK, TPCK and leupeptin caused a rapid and significant increase in vascular permeability. The TLCK- and TPCK-induced increase declined gradually, whereas leupeptin inhibited the vascular permeability after the temporary increase. When the inhibitors were injected 5 h after carrageenin injection, TLCK and TPCK increased the vascular permeability, whereas leupeptin was without effect. Cyproheptadine, an anti-histamine and antiserotonin drug, inhibited the leupeptin-induced temporary increase, but failed to inhibit the TLCK- and TPCK-induced increase in vascular permeability. These results suggest that the leupeptin-induced increase in vascular permeability was mediated by histamine and serotonin, while TLCK and TPCK may increase vascular permeability as a result of a direct action on endothelial cells.

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Abbreviations

TLCK:

N-α-p-tosyl-L-lysine chlorometyl ketone

TPCK:

L-1-tosylamide-2-phenylethyl chlorometyl ketone

F-BSA:

fluorescein isothiocyanate labeled bovine serum albumin

PBS:

phosphate-buffered saline

DMSO:

dimethylsulfoxide

DFP:

diisopropylfluorophosphate

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Author notes

  1. Kazuyoshi Watanabe & Hideo Nakagawa

    Present address: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani 2630, 930-01, Toyama, Japan

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, 980, Sendai, Japan

    Kazuyoshi Watanabe, Hideo Nakagawa & Susumu Tsurufuji

Authors
  1. Kazuyoshi Watanabe
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  2. Hideo Nakagawa
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  3. Susumu Tsurufuji
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Watanabe, K., Nakagawa, H. & Tsurufuji, S. Vascular permeability changes by proteinase inhibitors in carrageenin-induced inflammation in rats. Agents and Actions 17, 472–477 (1986). https://doi.org/10.1007/BF01965516

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  • DOI: https://doi.org/10.1007/BF01965516

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