Abstract
The human gastric epithelial cell line HGT-1 possesses adenylate cyclase-coupled histamine H2 receptors. To test the cellular homogeneity or heterogeneity with respect to these receptors, we have isolated 7 clones from the HGT-1 line and studied their basal and histamine-stimulated adenylate cyclase activities. Basal adenylate cyclase activities of the clones did not differ significantly, nor did 10 mM NaF-nor 0.1 mM Gpp(NH)p-stimulated activities. However, histamine stimulation of adenylate cyclase varied among clones from 1.9 fold to 5.4 fold basal activity. The EC50 values, determined in 3 clones, were not significantly different. These findings support the heterogeneity of histamine responsiveness of the human gastric cell line HGT-1. In addition, they suggest that highly histamine-responsive clones may be useful models to study the gastric histamine H2-receptor and its specific antagonists in the human.
Similar content being viewed by others
References
C.L. Laboisse, C. Augeron, M. H. Couturier-Turpin, C. Gespach, A.M. Cheret andF. Potet,Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H 2 receptors, Cancer Res.42, 1541–1548 (1982).
S. Emami, C. Gespach, C. Augeron, C. Laboisse andG. Rosselin, The established human gastric cancer cell line (HGT-1) as a model for studies on cell surface receptors in the human gastric epithelia. InGut Peptides and Ulcer, pp. 64–72 (Ed.A. Miyoshi). Biomedical Research Foundation, Tokyo 1983.
S. Emami, C. Gespach, M.E. Forgue-Lafitte, Y. Broer andG. Rosselin,Histamine and VIP interactions with receptor-cyclic AMP systems in the human gastric cancer cell line HGT-1, Life Sci.33, 415–423 (1983).
C.V. Perrier andM. Griessen,Action of H 1 and H 2 inhibitors on the response of histamine sensitive adenylyl cyclase from guinea-pig mucosa, Eur. J. Clin. Invest.6, 113–120 (1976).
S. Batzri andJ.D. Gardner,Action of histamine on cyclic AMP in guinea pig gastric cells: inhibition by H 1-and H 2-receptor antagonists, Mol. Pharmacol.16, 406–416 (1979).
A.M. Cheret, F. Pignal andM.J.M. Lewin,Effects of H 2-receptor antagonists cimetidine, ranitidine, and ICI 125,211 on histamine-stimulated adenylate cyclase activity in Guinea pig gastric mucosa. Mol. Pharmacol.20, 326–330 (1981).
G. H. Heppner,Tumor heterogeneity, Cancer Res.44, 2259–2265 (1984).
T.R. Chen,In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain, Exp. Cell Res.104, 255–262 (1977).
G.W. Snedecor andW.G. Cochran, InStatistical Methods Applied to Agriculture and Biology, pp. 251–254. I.S.U.P., U.S.A. 1956.
S. Siegel. InNonparametric Statistics for the Behavioral Sciences, pp. 184–194. McGraw-Hill Book Company, New York 1956.
R.J. Majeska, S.B. Rodan andG.A. Rodan,Parathyroid hormone-responsive clonal cell lines from rat osteosarcoma, Endocrinology107, 1494–1503 (1980).
J.R. Sheppard, T.P. Koestler, S.P. Corwin, C. Buscarino, J. Doll, B. Lester, R.G. Greig andG. Poste,Experimental metastasis correlates with cyclic AMP accumulation in B16 melanoma clones, Nature308, 544–547 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cheret, AM., Laboisse, C.L., Roumagnac, I. et al. Highly histamine-responsive clones from the human gastric adenocarcinoma cell line HGT-1. Agents and Actions 17, 436–440 (1986). https://doi.org/10.1007/BF01965510
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01965510